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https://www.arca.fiocruz.br/handle/icict/24925
SELECTING THE RIGHT GATE TO IDENTIFY RELEVANT CELLS FOR YOUR ASSAY: A STUDY OF THIOGLYCOLLATE-ELICITED PERITONEAL EXUDATE CELLS IN MICE
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Abstract
In this study, we investigate the diversity and modulation of leukocyte populations represented in the gates defined by size and granularity at different time points of thioglycollate-induced peritonitis in mouse. Results: The inflammatory cells were distributed into four regions (R1–R4) of a data plot graph defined by cell size
and granularity. R1 and R2 contained agranular cells that were small in size and predominately included T (
CD3+)
lymphocytes along with B (
B220+) lymphocytes. Macrophages (F4/80+) were the predominant cells found in the R3
region. However, these cells were present in all regions, albeit at a lower frequency in R1 and R2. Granulocytes (
Gr1+)
were mainly distributed in R3 and R4. The wide distribution of F4/80+ and Gr1+
cells may reflect the recruitment and
activation state of the different macrophage and granulocyte populations. Based on these observations, size and
granularity may contribute to an initial step in the analysis and sorting of thioglycollate-elicited peritoneal exudate
cells. However, the developmental stage and cell activation state may interfere with cell segregation using size and
granularity as parameters.
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