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https://www.arca.fiocruz.br/handle/icict/23368
MATERNAL SCHISTOSOMIASIS ALTERS COSTIMULATORY MOLECULES EXPRESSION IN ANTIGEN-PRESENTING CELLS FROM ADULT OFFSPRING MICE
Aleitamento Materno
Moléculas estimuladoras
Gravidez
Esquistossomose materna
imunologia
Antígenos CD
metabolismo
Imunidade Materno-Adquirida
Complicações Parasitárias na Gravidez
Esquistossomose mansoni
Animais
Antígenos CD40
Antígenos CD80
Antígenos CD86
Feminino
Transmissão Vertical de Doença Infecciosa
Masculino
Camundongos
Gravidez
Esquistossomose mansoni
transmissão
Author
Affilliation
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil / Federal University of Pernambuco. Health Sciences Center. Department of Tropical Medicine. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil / Federal University of Pernambuco. Health Sciences Center. Department of Tropical Medicine. Recife, PE, Brazil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil / Federal University of Pernambuco. Health Sciences Center. Department of Pharmaceutical Sciences. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil / Federal University of Pernambuco. Health Sciences Center. Department of Tropical Medicine. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil / Federal University of Pernambuco. Health Sciences Center. Department of Tropical Medicine. Recife, PE, Brazil.
Federal University of Pernambuco. Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA). Recife, PE, Brazil / Federal University of Pernambuco. Health Sciences Center. Department of Pharmaceutical Sciences. Recife, PE, Brazil.
Abstract
Adult offspring of Schistosoma mansoni-infected mice showed alterations in immunity to a heterologous antigen, ovalbumin (OA). Prior breastfeeding induced increased production of anti-OA antibodies, while pregnancy impaired it. Here, we investigated the expression of costimulatory molecules on antigen-presenting cells (APCs) of the adult offspring of S. mansoni-infected mothers in response to OA. Newborn mice were divided into three groups: animals Born Infected Mothers (BIM) suckled by non-infected mothers; animals from non-infected mothers Suckled Infected Mothers (SIM); and another group of mice born from and suckled by non-infected mothers (CONTROL). The adult offspring were immunized with subcutaneous OA+adjuvant, and 3-8days following immunization, double labeling was performed (CD45R/B220 or CD11c and CD80, CD86, CD40 or HLA-DR) on spleen cells. In comparison to the CONTROL group, an early increased frequency of CD40+/CD80+ B cells was observed in SIM mice (p<0.001/p<0.05), but no alteration of CD11c+ cells was observed. In contrast, in BIM mice, the frequency of CD86+/CD11c+ cells (p<0.05) and CD40+/CD80+/CD86+ B cells (p<0.01/p<0.01/p<0.05) was drastically reduced. In conclusion, previous suckling by S. mansoni-infected mothers enabled improved antigen presentation by B cells in adult offspring, whereas gestation in these mothers imprinted offspring with weak antigen presentation by APCs during the immune response to a non-related antigen.
Keywords in Portuguese
Crianças AdultasAleitamento Materno
Moléculas estimuladoras
Gravidez
Esquistossomose materna
DeCS
Células Apresentadoras de Antígenosimunologia
Antígenos CD
metabolismo
Imunidade Materno-Adquirida
Complicações Parasitárias na Gravidez
Esquistossomose mansoni
Animais
Antígenos CD40
Antígenos CD80
Antígenos CD86
Feminino
Transmissão Vertical de Doença Infecciosa
Masculino
Camundongos
Gravidez
Esquistossomose mansoni
transmissão
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