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2030-01-01
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2 Fome zero e agricultura sustentávelColecciones
- IOC - Artigos de Periódicos [12678]
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TWO NOVEL DEFENSIN-ENCODING GENES OF THE CHAGAS DISEASE VECTOR TRIATOMA BRASILIENSIS (REDUVIIDAE, TRIATOMINAE): GENE EXPRESSION AND PEPTIDE-STRUCTURE MODELING
Triatoma
Imunidade aos insetos
Alimentação de sangue
Defensinas
Insetos
Triatoma brasiliensis
Digestive tract
Insect immunity
Blood feeding
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Resumen en ingles
Defensins are cysteine-rich peptides involved in the innate immunity of insects and many other organisms. In the present study, two novel defensin-encoding cDNAs and the respective genomic DNAs (def3 and def4) of Triatoma brasiliensis were identified and their tissue-specific and temporal expression was characterized. Both of the deduced mature peptides consisted of 43 amino acid residues and were highly similar to previously identified triatomine defensins (81.4-100%). Semi-quantitative RT-PCR data showed that def3 was constitutively expressed in the fat body and was induced in salivary glands and the small intestine at 5 and 3 days after feeding (daf), respectively. The def4 mRNA level was highly up-regulated in the stomach and fat-body tissues at 5 and 3 daf, respectively. The three-dimensional structures of these defensins were predicted using a homology modeling approach with Def-AAA, the defensin from Anopheles gambiae, as template (62-74% identity). A map of the electrostatic potential of these models revealed that, despite their similar folding patterns, mature Def2 and Def4 have a more cationic structure than is the case for Def1 and Def3. Such differences may orient the antimicrobial profile of these defensins against distinct targets in different organs of the insect.
Palabras clave en portugues
Trato digestivoTriatoma
Imunidade aos insetos
Alimentação de sangue
Defensinas
Insetos
Palabras clave en ingles
DefensinTriatoma brasiliensis
Digestive tract
Insect immunity
Blood feeding
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