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ArtículoDerechos de autor
Acceso restringido
Fecha del embargo
2030-01-01
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- IOC - Artigos de Periódicos [12678]
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DEFICIENCY OF GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 1 DECREASES TRIACYLGLYCEROL STORAGE AND INDUCES FATTY ACID OXIDATION IN INSECT FAT BODY
Beta-oxidação
Metabolismo dos ácidos gordurosos
Síntese de triacilglicerol
Oxidação de ácido gordo
Fisiologia dos Insetos
Fatty acid metabolism
Lipids
Triacylglycerol synthesis
Fatty acid oxidation
Insect physiology
Autor
Afiliación
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / University of North Carolina. Department of Nutrition. Chapel Hill. USA.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
University of North Carolina. Department of Nutrition. Chapel Hill. USA / University of North Carolina. Department of Medicine. Chapel Hill, USA.
University of North Carolina. Department of Nutrition. Chapel Hill. USA.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
University of North Carolina. Department of Nutrition. Chapel Hill. USA / University of North Carolina. Department of Medicine. Chapel Hill, USA.
University of North Carolina. Department of Nutrition. Chapel Hill. USA.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
Glycerol-3-phosphate acyltransferases (GPAT) catalyze the initial and rate-limiting step for the de novo synthesis of triacylglycerol (TAG). Four mammalian GPAT isoforms have been identified: the mitochondria-associated GPAT1 and 2, and the endoplasmic reticulum (ER)-associated GPAT3 and 4. In the insect Rhodnius prolixus, a vector of Chagas' disease, we previously predicted a mitochondrial-like isoform (RhoprGPAT1) from genomic data. In the current study, we clone the RhoprGPAT1 coding sequence and identify an ER-associated GPAT (RhoprGPAT4) as the second isoform in the insect. RhoprGPAT1 contributes 15% of the total GPAT activity in anterior midgut, 50% in posterior midgut and fat body, and 70% in the ovary. The RhoprGpat1 gene is the predominant transcript in the midgut and fat body. To evaluate the physiological relevance of RhoprGPAT1, we generate RhoprGPAT1-deficient insects. The knockdown of RhoprGpat1 results in 50% and 65% decrease in TAG content in the posterior midgut and fat body, respectively. RhoprGpat1-deficient insects also exhibits impaired lipid droplet expansion and a 2-fold increase in fatty acid β-oxidation rates in the fat body. We propose that the RhoprGPAT1 mitochondrial-like isoform is required to channel fatty acyl chains towards TAG synthesis and away from β-oxidation. Such a process is crucial for the insect lipid homeostasis.
Palabras clave en portugues
LipídiosBeta-oxidação
Metabolismo dos ácidos gordurosos
Síntese de triacilglicerol
Oxidação de ácido gordo
Fisiologia dos Insetos
Palabras clave en ingles
Beta-oxidationFatty acid metabolism
Lipids
Triacylglycerol synthesis
Fatty acid oxidation
Insect physiology
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