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PERFORMANCE OF MOLECULAR METHODS FOR HEPATITIS C VIRUS DIAGNOSIS: USEFULNESS AMONG CHRONIC CASES AND DURING THE COURSE OF INFECTION
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Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Laboratório Central de Saúde Pública Noel Nutels. Divisão de Hepatites. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Laboratório Central de Saúde Pública Noel Nutels. Divisão de Hepatites. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Abstract
Background: Molecular methods are essential to define hepatitis C virus (HCV) infection. This study was conducted
to evaluate the performance of molecular qualitative and quantitative methods for HCV RNA among chronic
patients and individuals during the course of HCV infection.
Methods: Single serum samples were obtained from 82 HCV infected individuals where six of them donated serial
serum samples (n = 52) during the course of HCV infection. Qualitative (in-house RT-nested PCR and COBAS®
AMPLICOR HCV Test v2.0 and TMA) and quantitative (COBAS® AMPLICOR HCV Monitor Test v2.0 and
bDNA) techniques were employed.
Results: TMA presented the highest rate (87.8%) of HCV detection among qualitative tests and it was the most
sensitive for HCV RNA detection during the early and late phases of HCV infection. HCV RNA was quantified
among 56 samples and significant correlation was observed between the two assays (r 0.92; p < 0.0001).
Conclusions: It is concluded that both quantitative methods can be used among chronic and acute HCV cases, but
TMA was the most efficient for HCV qualitative detection among chronic cases and in the early and late phases of
HCV infection.
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