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https://www.arca.fiocruz.br/handle/icict/19419
SENSITIVITY OF RT-PCR METHOD IN SAMPLES SHOWN TO BE POSITIVE FOR ZIKA VIRUS BY RT-QPCR IN VECTOR COMPETENCE STUDIES
Autor(es)
Afiliação
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.
University of California-Davis. Department of Molecular and Cellular Biology. Davis, CA, USA.
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.
University of California-Davis. Department of Molecular and Cellular Biology. Davis, CA, USA.
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.
Resumo em Inglês
Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
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