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SENSITIVITY OF RT-PCR METHOD IN SAMPLES SHOWN TO BE POSITIVE FOR ZIKA VIRUS BY RT-QPCR IN VECTOR COMPETENCE STUDIES
Paiva, Marcelo Henrique Santos et al. | Fecha del documento: 2017
Autor
Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
Afiliación
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.
University of California-Davis. Department of Molecular and Cellular Biology. Davis, CA, USA.
Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil.
Resumen en ingles
Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
Palabras clave en ingles
ZIKV
RT-PCR
Aedes aegypti
Vector competence
 
Referencia
PAIVA, M. H. S. et al. Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies. Genetics and Molecular Biology, v. 40, n. 3, p. 597–599, 22 maio 2017.
DOI
10.1590/1678-4685-GMB-2016-0312
ISSN
1415-4757