Use este identificador para citar ou linkar para este item:
https://www.arca.fiocruz.br/handle/icict/19302
Tipo de documento
ArtigoDireito Autoral
Acesso aberto
Objetivos de Desenvolvimento Sustentável
03 Saúde e Bem-EstarColeções
Metadata
Mostrar registro completo
PERFORMANCE ASSESSMENT OF FOUR CHIMERIC TRYPANOSOMA CRUZI ANTIGENS BASED ON ANTIGEN-ANTIBODY DETECTION FOR DIAGNOSIS OF CHRONIC CHAGAS DISEASE
Antígenos , protozoários / imunologia
Doença de Chagas / diagnóstico
Ensaio de Imunoadsorção Enzimática / métodos
Trypanosoma cruzi / imunologia
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Resumo em Inglês
The performance of serologic tests in chronic Chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-Trypanosoma cruzi antibodies. Whole-cell T. cruzi extracts or recombinant proteins have shown variation in the performance and cross-reactivity. Synthetic chimeric proteins comprising fragments of repetitive amino acids of several different proteins have been shown to improve assay performances to detect Chagasic infections. Here, we describe the production of four chimeric T. cruzi proteins and the assessment of their performance for diagnostic purposes. Circular Dichroism spectra indicated the absence of well-defined secondary structures, while polydispersity evaluated by Dynamic Light Scattering revealed only minor aggregates in 50 mM carbonate-bicarbonate (pH 9.6), demonstrating that it is an appropriate buffering system for sensitizing microplates. Serum samples from T. cruzi-infected and non-infected individuals were used to assess the performance of these antigens for detecting antibodies against T. cruzi, using both enzyme-linked immunosorbent assay and a liquid bead array platform. Performance parameters (AUC, sensitivity, specificity, accuracy and J index) showed high diagnostic accuracy for all chimeric proteins for detection of specific anti-T. cruzi antibodies and differentiated seropositive individuals from those who were seronegative. Our data suggest that these four chimeric proteins are eligible for phase II studies.
DeCS
Anticorpos, protozoários / imunologiaAntígenos , protozoários / imunologia
Doença de Chagas / diagnóstico
Ensaio de Imunoadsorção Enzimática / métodos
Trypanosoma cruzi / imunologia
Compartilhar