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TRANSCRIPTOME PATTERNS FROM PRIMARY CUTANEOUS LEISHMANIA BRAZILIENSIS INFECTIONS ASSOCIATE WITH EVENTUAL DEVELOPMENT OF MUCOSAL DISEASE IN HUMANS
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Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA .
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Ambulatório de Leishmanioses. Rio de Janeiro, RJ, Brasil.
VA Medical Center West Los Angeles. Infectious Diseases Section (111 F) and Research Service. Los Angeles, CA, USA. / University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA.
University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA / UCLA-DOE Institute for Genomics and Proteomics. Crump Institute for Molecular Imaging. Department of Molecular and Medical Pharmacology. Los Angeles, CA, USA.
University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA / UCLA-DOE Institute for Genomics and Proteomics. Crump Institute for Molecular Imaging. Department of Molecular and Medical Pharmacology. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA.
Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA .
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Ambulatório de Leishmanioses. Rio de Janeiro, RJ, Brasil.
VA Medical Center West Los Angeles. Infectious Diseases Section (111 F) and Research Service. Los Angeles, CA, USA. / University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA.
University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA / UCLA-DOE Institute for Genomics and Proteomics. Crump Institute for Molecular Imaging. Department of Molecular and Medical Pharmacology. Los Angeles, CA, USA.
University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA / UCLA-DOE Institute for Genomics and Proteomics. Crump Institute for Molecular Imaging. Department of Molecular and Medical Pharmacology. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA.
Abstract
Introduction: Localized Cutaneous Leishmaniasis (LCL) and Mucosal Leishmaniasis (ML) are two extreme clinical forms of American Tegumentary Leishmaniasis that usually begin as solitary primary cutaneous lesions. Host and parasite factors that influence the progression of LCL to ML are not completely understood. In this manuscript, we compare the gene expression
profiles of primary cutaneous lesions from patients who eventually developed ML to those that did not. Methods: Using RNA-seq, we analyzed both the human and Leishmania transcriptomes in primary cutaneous lesions.
Results: Limited number of reads mapping to Leishmania transcripts were obtained. For human transcripts, compared to ML patients, lesions from LCL patients displayed a general multi-polarization of the adaptive immune response and showed upregulation of genes involved in chemoattraction of innate immune cells and in antigen presentation. We also identified a
potential transcriptional signature in the primary lesions that may predict long-term disease outcome. Conclusions: We were able to simultaneously sequence both human and Leishmania mRNA transcripts in primary
cutaneous leishmaniasis lesions. Our results suggest an intrinsic difference in the immune capacity of LCL and ML patients. The findings correlate the complete cure of L. braziliensis infection with a controlled inflammatory response and a balanced activation of innate and adaptive immunity.
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