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https://www.arca.fiocruz.br/handle/icict/1874
ANTIBODIES TO THE PLASMODIUM FALCIPARUM RHOPTRY PROTEIN RAP-2⁄RSP-2 IN RELATION TO ANAEMIA IN CAMEROONIAN CHILDREN
Complement
Malarial anaemia
Phagocytosis
Plasmodium falciparum
Rhoptry-associated protein- 2 (RAP-2 ⁄ RSP-2)
Author
Affilliation
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
University of Buea. Faculty of Health Sciences. Buea, Cameroon
Universidade Federal do Amazonas. Manaus, AM, Brazil
Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil
Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
Institute Pasteur. Host Pathogen Interactions Unit. Guadeloupe, Abymes Cedex, France
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
University of Buea. Faculty of Health Sciences. Buea, Cameroon
Universidade Federal do Amazonas. Manaus, AM, Brazil
Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil
Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
Institute Pasteur. Host Pathogen Interactions Unit. Guadeloupe, Abymes Cedex, France
Stockholm University. Wenner-Gren Institute. Department of Immunology. Stockholm, Sweden
Abstract
Previous studies have implicated reactive antibodies to the low
molecular weight rhoptry-associated proteins (RAP-1, RAP-2 ⁄
RSP-2 and RAP-3) in erythroid cell destruction during
Plasmodium falciparum infection. In this pilot study, the
frequency, specificity and functional capacity of naturally
acquired anti-RAP-2 ⁄ RSP-2 antibodies were investigated in
the sera of anaemic and nonanaemic malaria-infected Cameroonian
children. All sera recognized RAP-2 ⁄ RSP-2 by
FACS, irrespective of the clinical status of the subjects. However,
the anaemic children showed higher levels of IgG antibodies
than the nonanaemic group, while both groups showed
similar levels of IgM antibodies. Only few individuals had
detectable levels of RAP-2 ⁄ RSP-2-specific IgG1 and IgG3
subclass antibodies, while no IgG2 and IgG4 subclass antibodies
were detected in these subjects. By ELISA, the
anaemic group tended to show higher levels of antibodies to
RAP-2 ⁄ RSP-2 regarding all antibody classes tested, except
for IgG4 and IgE. Unexpectedly, sera from the nonanaemic
group activated complement to a greater extent than those
from the anaemic group. These results need to be confirmed in
extended studies but indicate that the effector functions of the
RAP-2 ⁄ RSP-2-reactive antibodies may be more important
than their amounts. Such antibodies could play a role in both
immunity and pathogenesis during P. falciparum infection.
Keywords
AntibodiesComplement
Malarial anaemia
Phagocytosis
Plasmodium falciparum
Rhoptry-associated protein- 2 (RAP-2 ⁄ RSP-2)
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