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A LACTATE DEHYDROGENASE ELISA-BASED ASSAY FOR THE IN VITRO DETERMINATION OF PLASMODIUM BERGHEI SENSITIVITY TO ANTI-MALARIAL DRUGS
Cultura in vitro
Infecção sincronizada
Ensaio in vitro com fármaco
Vacinas Antimaláricas
L-Lactato Desidrogenase
Ensaio de Imunoadsorção Enzimática
schizont
synchronized infection
drug in vitro assay
anti-malarials
IC50 values
lactate dehydrogenase
ELISA
Plasmodium berghei
Affilliation
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA.
La Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Abstract
Plasmodium berghei rodent malaria is a well-known model for the investigation of anti-malarial drug efficacy in vivo. However, the availability of drug in vitro assays in P. berghei is reduced when compared with the spectrum of techniques existing for Plasmodium falciparum. New alternatives to the current manual or automated methods described for P. berghei are attractive. The present study reports a new ELISA drug in vitro assay for P. berghei using two monoclonal antibodies against the parasite lactate dehydrogenase (pLDH).
Keywords in Portuguese
Plasmodium bergheiCultura in vitro
Infecção sincronizada
Ensaio in vitro com fármaco
Vacinas Antimaláricas
L-Lactato Desidrogenase
Ensaio de Imunoadsorção Enzimática
Keywords
In vitro cultureschizont
synchronized infection
drug in vitro assay
anti-malarials
IC50 values
lactate dehydrogenase
ELISA
Plasmodium berghei
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