Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations

Federal University of Piauí. Laboratory in Experimental Cancerology. Department of Biophysics and Physiology.Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, Brazil
Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Department of Pharmacy. Teresina, PI, Brazil
Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
State University of São Paulo. Chemistry Institute. Araraquara, SP, Brazil
State University of SãoPaulo. Center for Medical Nuclear. Radioisotopes Research Laboratory. São Paulo, SP, Brazil
State University of Paulo. São Faculty of Medicine. Department of Radiology. São Paulo, SP, Brazil
Federal University of Ceará. Department of Clinical Odontology. Fortaleza, CE, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil / Oswaldo Cruz Foundation. Fortaleza, CE, Brazil

Abstract

Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim ofthestudy: Toassessthe in vivoandexvivo antitumor actionofafractionwithcasearins(FC)andits main component-CasearinX-isolatedfrom C. sylvestris leaves. Materials andmethods: Firstly,Sarcoma180bearingSwissmiceweretreatedwithFCandCasXfor 7 days.Secondly,BALB/cnudeanimalsreceivedhollow fibers withcoloncarcinoma(HCT-116)orglio- blastoma (SF-295)cellsandweretreatedwithFCfor4days.On5thday,proliferationwasdeterminedby MTT assay. Results: FC 10and25mg/kg/dayi.p.and50mg/kg/dayoralandCasX25mg/kg/dayi.p.and50mg/kg/ day oralrevealedtumorgrowthinhibitionratesof35.8,86.2,53.7,90.0and65.5%andsuchtumors demonstratedraremitosesandcoagulationnecrosisareas.Similarly,FCreducedmultiplyingofHCT-116 and SF-295cellswhenevaluatedbytheHollowFiberAssay(2.5and5mg/kg/dayi.p.and25and50mg/ kg/day oral),withcellgrowthinhibitionratesrangingfrom33.3to67.4%(po0.05). Flowcytometry experimentsrevealedthatFCreducedmembraneintegrityandinducedDNAfragmentationandmi- tochondrial depolarization(po0.05). Conclusions: FC andCasXwereefficient antitumorsubstancesagainstmurineandhumancancercells and causedreversiblemorphologicalchangesinliver,kidneysandspleens,emphasizingclerodanedi- terpenes asanemergingclassofanticancermolecules.

Keywords in Portuguese

Casearin X
Teste de fibra oca
Sarcoma 180
Leucograma
Antineoplásico

Keywords

Casearin X
Hollow fiber assay
Sarcoma 180
Antineoplastic
Leukogram

Publisher

Elsevier

Citation

FERREIRA, P. M. P. et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270–279, 2016.

DOI

10.1016/j.jep.2016.04.011

ISSN

0378-8741

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18081

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