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https://www.arca.fiocruz.br/handle/icict/17751
HER-2 AND EGFR MRNA EXPRESSION AND ITS RELATIONSHIP WITH VERSICAN IN MALIGNANT MATRIX-PRODUCING TUMORS OF THE CANINE MAMMARY GLAND
Genes EGFR
Carcinoma
Proteínas
Tumores mamarios animais
Animais
Author
Affilliation
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. School of Veterinary Medicine and Zootechny. Department of Pathology and Clinics. Salvador, BA, Brazil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
International Center of Research in Cancer (CIPE). A.C. Camargo Cancer Center. São Paulo, SP, Brazil
International Center of Research in Cancer (CIPE). A.C. Camargo Cancer Center. São Paulo, SP, Brazil
Universidade Federal de Minas Gerais. School of Pharmacy. Department of Clinical and Toxicological Analyses. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. School of Veterinary Medicine and Zootechny. Department of Pathology and Clinics. Salvador, BA, Brazil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
International Center of Research in Cancer (CIPE). A.C. Camargo Cancer Center. São Paulo, SP, Brazil
International Center of Research in Cancer (CIPE). A.C. Camargo Cancer Center. São Paulo, SP, Brazil
Universidade Federal de Minas Gerais. School of Pharmacy. Department of Clinical and Toxicological Analyses. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Abstract
Versican expression promotes tumor growth by destabilizing focal cell contacts, thus impeding cell adhesion and facilitating cell migration. It not only presents or recruits molecules to the cell surface, but also modulates gene expression levels and coordinates complex signal pathways. Previously, we suggested that the interaction between versican and human epidermal growth factor receptors may be directly associated with tumor aggressiveness. Thus, the expression of EGFR and HER-2 in these neoplasms may contribute to a better understanding of the progression mechanisms in malignant mammary tumors. The purpose of this study was to correlate the gene and protein expressions of EGFR and HER2 by RNA In Situ Hybridization (ISH) and immunohistochemistry (IHC), respectively, and their relationship with the versican expression in carcinomas in mixed tumors and carcinosarcomas of the canine mammary gland. The results revealed that EGFR mRNA expression showed a significant difference between in situ and invasive carcinomatous areas in low and high versican expression groups. Identical results were observed in HER-2 mRNA expression. In immunohistochemistry analysis, neoplasms with low versican expression showed greater EGFR immunostaining in the in situ areas than in invasive areas, even as the group presenting high versican expression displayed greater EGFR and HER-2 staining in in situ areas. Significant EGFR and HER-2 mRNA and protein expressions in in situ carcinomatous sites relative to invasive areas suggest that these molecules play a role during the early stages of tumor progression.
Keywords in Portuguese
Glandulas mamarias de animaisGenes EGFR
Carcinoma
Proteínas
Tumores mamarios animais
Animais
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