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SECRETOME FROM RESIDENT CARDIAC STROMAL CELLS STIMULATES PROLIFERATION, CARDIOMYOGENESIS AND ANGIOGENESIS OF PROGENITOR CELLS
Cardiac resident stromal cells
Stem cells
Angiogenesis
Conditioned medium
Extracellular matrix
Células-Tronco
Indutores da Angiogênese
Meios de Cultivo Condicionados
Matriz Extracelular
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Santa Casa Hospital. Bank of Valve Homografts. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Santa Casa Hospital. Bank of Valve Homografts. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-Tronco. Curitiba, PR, Brasil.
Resumen en ingles
In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sources to induce cardiac lineage specification for maintenance of tissue homeostasis and repair. Cardiac resident stromal cells (CRSCs) may play a pivotal role in cardiac repair throughout their secretome. Here, we performed the characterization of CRSCs and their secretome by analyzing the composition of their culture-derived extracellular matrix (ECM) and conditioned medium (CM) and by investigating their potential effect on adipose-derived stem cell (ADSC) and progenitor cell behavior. We confirmed that CRSCs are a heterogeneous cell population whose secretome is composed by proteins related to cellular growth, immune response and cardiovascular development and function. We also observed that CRSC secretome was unable to change the behavior of ADSCs, except for proliferation. Additionally, CM from CRSCs demonstrated the potential to drive proliferation and cardiac differentiation of H9c2 cells and also the ability to induce angiogenesis in vitro. Our data suggest that the CRSCs can be a source of important modulating signals for cardiac progenitor cell recruitment/activation.
Palabras clave en ingles
SecretomeCardiac resident stromal cells
Stem cells
Angiogenesis
Conditioned medium
Extracellular matrix
DeCS
Células EstromaisCélulas-Tronco
Indutores da Angiogênese
Meios de Cultivo Condicionados
Matriz Extracelular
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