Use este identificador para citar ou linkar para este item:
https://www.arca.fiocruz.br/handle/icict/16124
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2030-01-01
Coleções
- IOC - Artigos de Periódicos [12642]
Metadata
Mostrar registro completo
CLONAL ANALYSIS OF HEPATITIS B VIRUSES AMONG BLOOD DONORS FROM JOINVILLE, BRAZIL: EVIDENCE OF DUAL INFECTIONS, INTRAGENOTYPE RECOMBINATION AND MARKERS OF RISK FOR HEPATOCELLULAR CARCINOMA
Progressão da doença
pré-S deleções
Resistência aos medicamentos
pre-S deletions
F141L
disease progression
transmitted drug resistance
Autor(es)
Afiliação
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Universidade da Região de Joinville. Departamento de Medicina. Joinville, SC, Brasil.
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Universidade da Região de Joinville. Departamento de Medicina. Joinville, SC, Brasil.
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
Hepatitis B virus (HBV) is classified into seven major genotypes (A-H). Brazil, a country of continental proportions, has three prevailing lineages of HBV genotypes A, D, and F. Distinct HBV genotypes have been associated with differential risk of disease progression. Pre-S gene deletions and single nucleotide polymorphisms have also been linked to progression to liver diseases. In this study, the molecular epidemiology of HBV was examined in Southern Brazil. The occurrence of multiple HBV infections, HBV recombination, and genetic markers of disease progression were also evaluated. Seventy-eight persons infected with HBV had their viruses characterized molecularly by nested PCR, DNA sequencing, and phylogenetic inference. Multiple infections and recombinant viruses were evaluated by clonal and bootscanning analyses. The vast majority (96%) of the strains belonged to different D subgenotypes. Three of the four strains with unresolved genotypic classification showed evidence of dual infections with distinct D subgenotypes by clonal analysis. There was also evidence of intragenotype mosaic viruses. While four viruses had pre-S deletions as major variants, another two displayed minor variants with such characteristics. One strain carried the F141L mutation, associated recently with increased risk of hepatocellular carcinoma. These results emphasize the need for monitoring HBV genotype distribution around South America, as well as for the presence of genetic markers of disease progression in subjects diagnosed with HBV recently.
Palavras-chave
Recombinação intra genótipoProgressão da doença
pré-S deleções
Resistência aos medicamentos
Palavras-chave em inglês
intragenotype recombinationpre-S deletions
F141L
disease progression
transmitted drug resistance
Compartilhar