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https://www.arca.fiocruz.br/handle/icict/14966
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2030-01-01
Coleções
- IOC - Artigos de Periódicos [12642]
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ANTI-HERPESVIRUS BOVINE TYPE 5 ACTIVITIES OF EXTRACTS OBTAINED FROM PLOCAMIUM BRASILIENSE
Bovine herpesvirus type 5
Monoterpenes
Attachment inhibition
Autor(es)
Afiliação
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil.
Universidade Federal Fluminense. Programa de Pós-Graduação em Química. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil.
Universidade Federal Fluminense. Programa de Pós-Graduação em Química. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI). Niterói, RJ, Brasil.
Resumo em Inglês
Bovine herpesvirus type 5 (BoHV-5) is an important
etiologic agent of meningoencephalitis in cattle and has
been frequently identified in outbreaks of neurological disease
in bovine in the southern hemisphere including Brazil. This
study aimed to evaluate the cytotoxic effect and the antiviral
properties of extracts obtained from Plocamium brasiliense
(Greville) Howe and Taylor in BoHV-5 RJ42/01 replication.
The cytotoxic effects were measured in Madin-Darbin bovine
kidney cells (MDBK) and cytotoxic concentration (CC50)
values have been determined for acyclovir (ACV) (223 μg±
2.0), ethyl acetate extract from P. brasiliense (2,109 μg±10),
hexane extract from P. brasiliense (7.181 μg±5), dichloromethane
extract from P. brasiliense (2.356 μg±6.5), and
hydroalcoholic extract from P. brasiliense (1.408 μg±5.8).
As a first approach to characterize the action of these extracts
on BoHV-5 RJ42/01, a virucidal assay activity was performed.
A virus suspension containing 1×105 plaqueforming
units (PFU) of the BoHV-5 RJ42/01 was mixed with
600 μg of extract and acyclovir and kept at room temperature
(24 °C) for 3 h. Meanwhile, a control of untreated infected
virus was performed in the same conditions. Then, treated
virus suspension and untreated control were diluted, and percentage
of inhibition of infectivity was determined by plaque
assay: ethyl acetate extract (99 %), hexane extract (90 %),
dichloromethane extract (99 %), and hydroalcoholic extract
(27 %). Acyclovir had a slight virucidal activity on viral
particle. The inhibition of attachment was performed in
MDBK cells inoculated with 100 PFU of BoHV-5 RJ42/01
in the presence or absence of various concentrations of extracts
(0.3, 0.9, and 1.5 μg mL−1
). Acyclovir was also assayed
at 2.8 and 11.25 μg mL−1
. The inhibition of adsorption was
also tested in MDBK cells treated with the same concentrations
of the extracts before virus inoculation. Results: hexane
extracts inhibited virus attachment in pre-treated cell 0.9 μg
(55 %) and 1.5 μg (71 %) and untreated MDBK cell only with
1.5 μg (63 %). Ethyl acetate extract on cell pre-treated with
0.3 μg (67 %), 0.9 μg (81 %), and 1.5 μg (91 %). Ethyl acetate
extract on pre-treated cell 0.3 μg (67 %), 0.9 μg (81 %), and
1.5 (91 %) but discrete inhibition on cell untreated. Dichloromethane
extract and acyclovir slightly inhibited virus binding
on MDBK cell.
Palavras-chave em inglês
Plocamium brasilienseBovine herpesvirus type 5
Monoterpenes
Attachment inhibition
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