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EFFECT OF AGE ON CUTICULAR HYDROCARBON PROFILES IN ADULT CHRYSOMYA PUTORIA (DIPTERA: CALLIPHORIDAE)
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Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Educação Ambiental e em Saúde. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Severino Sombra. Mestrado Profissional em Ciências Ambientais. Vassouras, RJ, Brasil.
University of Nevada. CABNR. Department of Biochemistry and Molecular Biology. Reno, North Virginia, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Educação Ambiental e em Saúde. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Severino Sombra. Mestrado Profissional em Ciências Ambientais. Vassouras, RJ, Brasil.
University of Nevada. CABNR. Department of Biochemistry and Molecular Biology. Reno, North Virginia, USA.
Abstract
A species-specific complex mixture of highly stable cuticular hydrocarbons (CHCs) covers the external
surface of all insects. Components can be readily analyzed by gas chromatography coupled to mass
spectrometry (GC–MS)to obtain a cuticular hydrocarbon profile, which may be used as an additionaltool
for the taxonomic differentiation of insect species and also for the determination of the age and sex of
adult and immature forms. We used GC–MS to identify and quantify the CHCs of female and male
Chrysomya putoria (Wiedemann, 1818) (Diptera: Calliphoridae) from one to five days old. CHCs ranged
from C21 to C35 for females and from C21 to C37 in males. Major compounds were the same for both
sexes and were 2-MeC28, C29:1, n-C29, 15-,13-MeC29, 2-MeC30, C31:1, n-C31 and 15-,13-MeC31. The
relative abundance of each component, however, varied with age. Cluster Analysis using Bray–Curtis
measure for abundance showed that cuticular hydrocarbon profiles are a strong and useful tool for the
determination of age in adult C. putoria.
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