Use este identificador para citar ou linkar para este item:
Tipo de documento
ArtigoDireito Autoral
Acesso aberto
Data de embargo
2016-12-30
Coleções
Metadata
Mostrar registro completo
EFFECT OF L-AMINO ACID OXIDASE FROM CALLOSELASMA RHODOSTHOMA SNAKE VENOM ON HUMAN NEUTROPHILS
Autor(es)
Afiliação
Resumo em Inglês
The in vitro effects of LAAO, an L-amino acid oxidase isolated from Calloselasma rhodosthoma snake venom, on isolated human neutrophil function were investigated. LAAO showed no toxicity on neutrophils. At non-cytotoxic concentrations, LAAO induced the superoxide anion production by isolated human neutrophil. This toxin, in its native form, is also able to stimulate the production of hydrogen peroxide in neutrophils, suggesting that its primary structure is essential for stimulation the cell. Moreover, the incubation of LAAO
and phenol red medium did not induce the production of hydrogen peroxide. Furthermore, LAAO was able to stimulate neutrophils to release proinflammatory mediators such as IL-8 and TNF-a as well as NETs liberation. Together, the data showed that the LAAO triggers relevant proinflammatory events. Particular regions of the molecule distinct from the LAAO catalytic site may be involved in the onset of inflammatory events.
Compartilhar