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https://www.arca.fiocruz.br/handle/icict/12078
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ArtigoDireito Autoral
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- IOC - Artigos de Periódicos [12747]
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A SIMPLE HEAT DISSOCIATION METHOD INCREASES SIGNIFICANTLY THE ELISA DETECTION SENSITIVITY OF THE NONSTRUCTURAL-1 GLYCOPROTEIN IN PATIENTS INFECTED WITH DENV TYPE-4
NS1 ELISA
Immune-complex dissociation
DENV-4
secondary infections
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
The secreted form of the dengue virus (DENV) nonstructural-1 (NS1) glycoprotein has been shown to be
useful for the diagnosis of DENV infections in patients’ serum samples. In a number of studies, the sensitivity
of the commercially available DENV NS1 glycoprotein detection assays was higher against some
DENV serotypes (DENV-1 > DENV-3 > DENV-2 = DENV-4) than others and were also lower using patients’
serum samples with secondary versus primary DENV infections. In this study, 471 DENV-4 positive acute
phase patients’ serum samples were selected from a large panel collected in Brazil from March 2011
to October 2012 by RT-PCR and/or virus isolation followed by serotype determination. The sera from
primary (n = 228) and secondary (n = 238) DENV-4 infections were identified using IgM and IgG capture
ELISAs. The sensitivity of a commercial DENV NS1 glycoprotein detection ELISA was then assessed when
these serum samples were not pre-treated or pre-treated by acid or heat dissociation prior to being tested.
Acid and heat dissociation of patients’ serum samples with primary and secondary DENV-4 infections
increased significantly the sensitivity of the DENV NS1 glycoprotein detection ELISA from 54.4% to 77.2%
(p < 0.05) and 82% (p < 0.05) and from 39.1% to 63.9% (p < 0.05) and 73.1% (p < 0.05), respectively. Treatment
of DENV infected patients’ serum samples using simple and rapid heat dissociation step (100 ◦C for
5 min) was, therefore, shown to be very useful for increasing the sensitivity of the DENV NS1 glycoprotein
detection ELISA using serum samples from either primary or secondary DENV infected patients.
Palavras-chave
DenguePalavras-chave em inglês
Dengue diagnosisNS1 ELISA
Immune-complex dissociation
DENV-4
secondary infections
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