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ILHEUS VIRUS ISOLATION IN THE PANTANAL, WEST-CENTRAL BRAZIL
Brazilian Pantanal
Wildlife species
Zoonotic arboviruses
Flaviviruses
ILHV RNA
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil / Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA / Fulbright Visiting Researcher in Doctorate Sandwich Program at CDC. Fort Collins, Colorado, USA.
Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA .
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Embrapa Pantanal. Corumbá, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA .
Fulbright Visiting Researcher in Doctorate Sandwich Program at CDC. Fort Collins, Colorado, USA.
Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA .
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Embrapa Pantanal. Corumbá, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA .
Fulbright Visiting Researcher in Doctorate Sandwich Program at CDC. Fort Collins, Colorado, USA.
Abstract
The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods,
conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolaˆndia sub-region of Pantanal
reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age
of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009.
To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region.
Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for
flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes
scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of
2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The
present data confirm the circulation of ILHV in the Brazilian Pantanal.
Keywords
Hematophagous arthropodsBrazilian Pantanal
Wildlife species
Zoonotic arboviruses
Flaviviruses
ILHV RNA
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