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A DECADE OF G3P[8] AND G9P[8] ROTAVIRUSES IN BRAZIL: EPIDEMIOLOGY AND EVOLUTIONARY ANALYSES
Group A rotaviruses
Genotypes G3 and G9
Phylogenetic analysis
Monovalent vaccine
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Clinical and Epidemiological. Leuven, Belgium.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Clinical and Epidemiological. Leuven, Belgium.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
This study aims to estimate the frequency of group A rotaviruses (RVA) infection with genotypes G3P[8]
and G9P[8] in children that suffered from diarrheal disease (DD) between 2001 and 2011 in different
Brazilian regions. In addition, the genetic diversity of G3P[8] and G9P[8] RVA strains recovered from vaccinated
and non-vaccinated children was assessed. Laboratory-based RVA surveillance included 15,115
cases of DD, and RVA was detected by enzyme immune-assay and/or polyacrylamide gel electrophoresis
in 3357 (22%) samples. RVA was genotyped by the semi-nested RT-PCR and among RVA-positive samples,
100 (2.9%) were G3 (63 G3P[8], 32 G3P not typed [NT], and 5 G3P[6]) and 378 (16.2%) were G9 (318
G9P[8], 59 G9P[NT], and 1 G9P[6]). From the G3 and G9 positive samples, 16 and 12, respectively, were
obtained from children aged 4–48 months vaccinated with the monovalent vaccine (Rotarix , RV1). Phylogenetic
analyses of the VP7 and VP8⁄ encoding genes were performed for 26 G3P[8] and 48 G9P[8]
strains. VP8⁄ phylogenetic analysis revealed that all strains analyzed belonged to P[8] lineage III, whereas
RV1 belongs to P[8]-I lineage. VP7 analysis revealed that all G3 and G9 strains belonged to G3-lineage III
and G9-lineage III. The comparison of the VP7 and VP8⁄ antigenic epitopes regions of Brazilian strains
with RV1 strain revealed several amino acid changes. However, no particular differences among Brazilian
strains detected before and after vaccine introduction were observed, or among strains detected from
vaccinated and non-vaccinated children. Complete genome characterization of four G3P[8] and seven
G9P[8] strains revealed a typical conserved human Wa-like genomic constellation. Changes in the genetic
diversity of G3P[8] and G9P[8] RVA detected from 2001 to 2011 in Brazil seemed not be related to RV1
introduction in Brazil.
Palabras clave en ingles
Diarrheal diseaseGroup A rotaviruses
Genotypes G3 and G9
Phylogenetic analysis
Monovalent vaccine
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