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Objetivos de Desenvolvimento Sustentável
06 Água potável e saneamentoColeções
- IOC - Artigos de Periódicos [12835]
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DETECTION OF ENTERIC VIRUSES IN ACTIVATED SLUDGE BY FEASIBLE CONCENTRATION METHODS
Sewage sludge
Activated sludge process
Virus concentration methods
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
Human enteric viruses are responsible to cause several diseases, including gastroenteritis and hepatitis,
and can be present in high amounts in sewage sludge. This study compared virus recovery efficiency
of two feasible concentration methods used for detecting human adenovirus (HAdV),
rotavirus species A (RV-A), norovirus genogroup II (NoV GII) and hepatitis A virus (HAV) in sewage
sludge from an activated sludge process. Twelve sewage sludge samples were collected
bi-monthly from January to July, 2011. Ultracentrifugation was compared with a simplified protocol
based on beef extract elution for recovering enteric viruses. Viruses were quantified by quantitative
real-time PCR assays and virus recovery efficiency and limits of detection were determined. Methods
showed mean recovery rates lower than 7.5%, presenting critical limits of detection (higher than 102 -
103 genome copies - GC L-1 for all viruses analyzed). Nevertheless, HAdV were detected in 90% of
the analyzed sewage sludge samples (range: 1.8 x 104 to 1.1 x 105 GC L-1), followed by RV-A and
NoV (both in 50%) and HAV (8%). Results suggesting that activated sludge is contaminated with
high viral loads and HAdV are widely disseminated in these samples. The low virus recovery rates
achieved, especially for HAV, indicate that other feasible concentration methods could be developed
to improve virus recovery efficiency in these environmental matrices.
Palavras-chave em inglês
Enteric virusesSewage sludge
Activated sludge process
Virus concentration methods
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