Author | Vieira, Angélica Thomaz | |
Author | Pinho, Vanessa | |
Author | Lepsch, Lucilia Brochado | |
Author | Scavone, Cristoforo | |
Author | Ribeiro, Ivone Maria | |
Author | Tomassini, Therezinha | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Soares, Milena Botelho Pereira | |
Author | Teixeira, Mauro Martins | |
Access date | 2015-04-14T14:03:28Z | |
Available date | 2015-04-14T14:03:28Z | |
Document date | 2005 | |
Citation | VIEIRA, A. T. et al. Mechanisms of the anti-inflammatory effects of the natural secosteroids physalins in a model of intestinal ischaemia and reperfusion injury. British Journal of Pharmacology, v.146, n. 2, p. 244-251, 2005. | pt_BR |
ISSN | 0007-1188 | |
ISSN | 10.1038/sj.bjp.0706321 | |
URI | https://www.arca.fiocruz.br/handle/icict/9989 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | open access | pt_BR |
Title | Mechanisms of the anti-inflammatory effects of the natural secosteroids physalins in a model of intestinal ischaemia and reperfusion injury. | pt_BR |
Type | Article | pt_BR |
Abstract | Reperfusion of an ischaemic tissue is associated with an intense inflammatory response and inflammation-mediated tissue injury. Physalins, a group of substances with secosteroidal chemical structure, are found in Physalis angulata stems and leaves. Here, we assessed the effects of physalins on the local, remote and systemic injuries following intestinal ischaemia and reperfusion (I/R) in mice and compared with the effects of dexamethasone. Following I/R injury, dexamethasone (10 mg kg(-1)) or physalin B or F markedly prevented neutrophil influx, the increase in vascular permeability in the intestine and the lungs. Maximal inhibition occurred at 20 mg kg(-1). Moreover, there was prevention of haemorrhage in the intestine of reperfused animals. Dexamethasone or physalins effectively suppressed the increase in tissue (intestine and lungs) and serum concentrations of TNF-alpha. Interestingly, treatment with the compounds was associated with enhancement of IL-10. The anti-inflammatory effects of dexamethasone or physalins were reversed by pretreatment with the corticoid receptor antagonist RU486 (25 mg kg(-1)). The drug compounds suppressed steady-state concentrations of corticosterone, but did not alter the reperfusion-associated increase in levels of corticosterone. The IL-10-enhancing effects of the drugs were not altered by RU486. In conclusion, the in vivo anti-inflammatory actions of physalins, natural steroidal compounds, appear to be mostly due to the activation of glucocorticoid receptors. Compounds derived from these natural secosteroids may represent novel therapeutic options for the treatment of inflammatory diseases. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunofarmacologia. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunofarmacologia. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Laboratorio de Neurofarmacologia Molecular. Departamento de Farmacologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Laboratorio de Neurofarmacologia Molecular. Departamento de Farmacologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunofarmacologia. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Reperfusion injury | pt_BR |
Subject | Inflammatory response | pt_BR |
Subject | TNF-a | pt_BR |
Subject | Glucocorticoids receptor | pt_BR |
Subject | IL-10 | pt_BR |
DeCS | Anti-Inflamatórios não Esteroides/uso terapêutico | pt_BR |
DeCS | Enteropatias/prevenção & controle | pt_BR |
DeCS | Lactonas/uso terapêutico | pt_BR |
DeCS | Traumatismo por Reperfusão/prevenção & controle | pt_BR |
DeCS | Esteroides/uso terapêutico | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Anti-Inflamatórios/farmacologia | pt_BR |
DeCS | Permeabilidade Capilar/efeitos de drogas | pt_BR |
DeCS | Quimiocinas/biossíntese | pt_BR |
DeCS | Citocinas/biossíntese | pt_BR |
DeCS | Dexametasona/farmacologia | pt_BR |
DeCS | Relação Dose-Resposta a Droga | pt_BR |
DeCS | Hemorragia Gastrointestinal/prevenção & controle | pt_BR |
DeCS | Glucocorticoides/farmacologia | pt_BR |
DeCS | Hemoglobinas/metabolismo | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Peroxidase/metabolismo | pt_BR |