Author | Rodrigues, Maurício Martins | |
Author | Soares, Milena Botelho Pereira | |
Author | Vasconcelos, José Ronnie Carvalho de | |
Access date | 2015-04-08T13:50:04Z | |
Available date | 2015-04-08T13:50:04Z | |
Document date | 2002 | |
Citation | RODRIGUES, M. M.; SOARES, M. B. P.; VASCONCELOS, J. R. C. Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization. Journal of Interferon Cytokine Research, v. 22, n. 11, p. 1137-1141, 2002. | pt_BR |
ISSN | 1079-9907 | |
URI | https://www.arca.fiocruz.br/handle/icict/9915 | |
Language | eng | pt_BR |
Publisher | Mary Ann Liebert, Inc | pt_BR |
Rights | open access | pt_BR |
Title | Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization. | pt_BR |
Type | Article | pt_BR |
Abstract | Intramuscular (i.m.) administration of eukaryotic plasmid vectors containing foreign genes is a general immunization
strategy capable of inducing protective type 1 immune responses against viral, bacterial, fungal,
and parasitic infections. We have described that immunization with a plasmid containing a gene encoding a
parasite antigen elicits specific type 1 protective immune responses against experimental infection with the
human protozoan parasite Trypanosoma cruzi. However, we had evidence suggesting that DNA immunization
concomitantly activated specific type 2 immune responses. To determine precisely the influence of the type 2
cytokine interleukin-4 (IL-4) during DNA immunization, we compared the immune responses of genetically
modified IL-4-deficient or wild-type (wt) BALB/c mice. IL-4-deficient mice had a significantly lower ratio of
specific serum IgG1/IgG2a, and on in vitro restimulation with antigen, their spleen cells secreted significantly
higher amounts of interferon-g (IFN-g). In contrast, absence of IL-4 did not affect total serum antibody response,
T cell proliferative responses, or activation of IFN-g-producing CD81 T cells. Our results suggested
that in contrast to conventional adjuvants, such as alum and complete Freund’s adjuvant, specific IgG1 in
DNA-immunized BALB/c mice was highly dependent on IL-4. To our knowledge, our study provides the first
evidence that endogenous IL-4 selectively downregulates the type 1 CD41 T cell-mediated immune response
induced by i.m. genetic immunization, a fact that may have implications for the design of certain DNA vaccines. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil | pt_BR |
DeCS | Linfócitos T CD4-Positivos/imunologia | pt_BR |
DeCS | Interleucina-4/imunologia | pt_BR |
DeCS | Vacinas de DNA/farmacologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Linfócitos T CD4-Positivos/efeitos de drogas | pt_BR |
DeCS | Imunidade Celular/efeitos de drogas | pt_BR |
DeCS | Imunoglobulina G/sangue | pt_BR |
DeCS | Injeções Intramusculares | pt_BR |
DeCS | Interleucina-4/deficiência | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |
DeCS | Camundongos Knockout | pt_BR |
DeCS | Baço/imunologia | pt_BR |
DeCS | Vacinas de DNA/administração & dosagem | pt_BR |