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https://www.arca.fiocruz.br/handle/icict/9494
CHROMATOGRAPHIC FINGERPRINT ANALYSIS AND EFFECTS OF THE MEDICINAL PLANT SPECIES MITRACARPUS FRIGIDUS ON ADULT SCHISTOSOMA MANSONI WORMS
High Pressure Liquid/methods
Plant Extracts/chemistry
Schistosoma mansoni/drug effects
Spleen/drug effects
Author
Affilliation
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Fundação Oswaldo Cruz. Rene Rachou Research Center. Schistosomiasis Laboratory. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Rene Rachou Research Center. Schistosomiasis Laboratory. Belo Horizonte, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Morphology. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Protein Structure and Function Study Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Protein Structure and Function Study Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Fundação Oswaldo Cruz. Rene Rachou Research Center. Schistosomiasis Laboratory. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Rene Rachou Research Center. Schistosomiasis Laboratory. Belo Horizonte, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Morphology. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Protein Structure and Function Study Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Protein Structure and Function Study Laboratory. Juiz de Fora, MG, Brazil
Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
Abstract
The aims of this work were to evaluate the in vitro and in vivo schistosomicidal properties of the methanolic extract of the aerial parts of Mitracarpus frigidus (MFM) and to determine its HPLC profile. For the in vitro experiment, four pairs of adult worms, obtained from infected mice, were exposed to different concentrations of MFM (100 to 400 𝜇g/mL) for 24 and 48 h and analyzed under an inverted microscope. For the in vivo experiment, mice were inoculated with cercariae and, 20 days after infection, MFM (100 and 300mg/kg) was administered orally for the following 25 days. Mice were euthanized after 60 days. MFM showed in vitro schistosomicidal activity, exhibiting the opening of the gynaecophoral canal of some male schistosomes, the presence of contorted muscles, vesicles, and the darkening of the paired worms skin. In vivo experiments showed that MFM treatments significantly reduced total wormcount, as praziquantel, showing a decrease in liver and spleen weight. Also, a significant reduction in granuloma density was observed. MFM treatment did not cause alterations in the liver function of either infected or noninfected mice. The HPLC chromatogram profile showed the presence of kaempferol-O-rutinoside, rutin, kaempferol, psychorubrin, and ursolic acid.
Keywords
ChromatographyHigh Pressure Liquid/methods
Plant Extracts/chemistry
Schistosoma mansoni/drug effects
Spleen/drug effects
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