Author | Niehus, Sebastian | |
Author | Smith, Terry K. | |
Author | Azzouz, Nahid | |
Author | Campos, Marco Antônio Silva | |
Author | Dubremetz, Jean François | |
Author | Gazzinelli, Ricardo Tostes | |
Author | Schwarz, Ralph T. | |
Author | Grockiego, Françoise Debierre | |
Access date | 2015-02-04T11:32:14Z | |
Available date | 2015-02-04T11:32:14Z | |
Document date | 2014 | |
Citation | NIEHUS, Sebastian et al. Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages. PLoS One, v. 9, n. 1, p. 1-11, 2014. | pt_BR |
ISSN | 1553-7366 | |
URI | https://www.arca.fiocruz.br/handle/icict/9423 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0085386. eCollection 2014 | |
Abstract | Glycosylphosphatidylinositols (GPIs) from several protozoan parasites are thought to elicit a detrimental stimulation of the host innate immune system aside their main function to anchor surface proteins. Here we analyzed the GPI biosynthesis of an avirulent Toxoplasma gondii type 2 strain (PTG) by metabolic radioactive labeling. We determined the biological function of individual GPI species in the PTG strain in comparison with previously characterized GPI-anchors of a virulent strain (RH). The GPI intermediates of both strains were structurally similar, however the abundance of two of six GPI intermediates was significantly reduced in the PTG strain. The side-by-side comparison of GPI-anchor content revealed that the PTG strain had only ∼ 34% of the protein-free GPIs as well as ∼ 70% of the GPI-anchored proteins with significantly lower rates of protein N-glycosylation compared to the RH strain. All mature GPIs from both strains induced comparable secretion levels of TNF-α and IL-12p40, and initiated TLR4/MyD88-dependent NF-κBp65 activation in macrophages. Taken together, these results demonstrate that PTG and RH strains differ in their GPI biosynthesis and possess significantly different GPI-anchor content, while individual GPI species of both strains induce similar biological functions in macrophages. | pt_BR |
Affilliation | Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany /Institute of Molecular and Cellular Biology. Strasbourg, France. | pt_BR |
Affilliation | University of St Andrews. Biomedical Sciences Research Complex. St Andrews, United Kingdom. | pt_BR |
Affilliation | Philipps University. Institute for Virology.Laboratory of Parasitology. Marburg, Germany. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | University of Montpellier. Montpellier, France. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG,Brazil / University of Massachusetts Medical School. Department of Medicine. Division of Infectious Diseases and Immunology. Worcester, MA, USA. | pt_BR |
Affilliation | Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/Lille 1 University. Unit of Structural and Functional Glycobiology. Lille, France. | pt_BR |
Affilliation | Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/ University of Tours. Infectiology and Public Health. Tours, France. | pt_BR |
Subject | Toxoplasma gondii | pt_BR |
Subject | Cercopithecus aethiops | pt_BR |
Subject | Glycosylphosphatidylinositols/metabolism | pt_BR |
Subject | Toxoplasma/pathogenicity | pt_BR |
Subject | Tumor Necrosis Factor-alpha/metabolism | pt_BR |