Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/9029
Title: Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
Authors: Rigato, Paula Ordonhez
Bargieri, Bruna Cunha de Alencar
Vasconcelos, Jose Ronnie Carvalho de
Dominguez, Mariana Ribeiro
Araujo, Adriano Fernando
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Rodrigues, Mauricio Martins
Affilliation: Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil/University of Massachusetts Medical School. Department of Medicine. Division of Infectious Disease and Immunology. Worcester, MA, USA
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular/ Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilRIGATO, Paula Ordonhez et al. Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi. Infect Immun, v. 79, n. 5, p. 2120-2130, 2011.
Abstract: Recently, we described a heterologous prime-boost strategy using plasmid DNA followed by replication defective human recombinant adenovirus type 5 as a powerful strategy to elicit long-lived CD8 T-cellmediated protective immunity against experimental systemic infection of mice with a human intracellular protozoan parasite, Trypanosoma cruzi. In the present study, we further characterized the protective long-lived CD8 T cells. We compared several functional and phenotypic aspects of specific CD8 T cells present 14 or 98 days after the last immunizing dose and found the following: (i) the numbers of specific cells were similar, as determined by multimer staining or by determining the number of gamma interferon (IFN-)-secreting cells by enzyme-linked immunospot (ELISPOT) assay; (ii) these cells were equally cytotoxic in vivo; (iii) following in vitro stimulation, a slight decline in the frequency of multifunctional cells (CD107a IFN- or CD107a IFN- tumor necrosis factor alpha positive [TNF-]) was paralleled by a significant increase of CD107a singly positive cells after 98 days; (iv) the expression of several surface markers was identical, except for the reexpression of CD127 after 98 days; (v) the use of genetically deficient mice revealed a role for interleukin-12 (IL-12)/IL-23, but not IFN-, in the maintenance of these memory cells; and (vi) subsequent immunizations with an unrelated virus or a plasmid vaccine or the depletion of CD4 T cells did not significantly erode the number or function of these CD8 T cells during the 15-week period. From these results, we concluded that heterologous plasmid DNA prime-adenovirus boost vaccination generated a stable pool of functional protective long-lived CD8 T cells with an effector memory phenotype.
Keywords: heterologous
Trypanosoma cruzi
Issue Date: 2011
Publisher: American Society for Microbiology
ISSN: 0019-9567
Copyright: open access
Appears in Collections:CPqRR - Artigos de Periódicos



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.