Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/8955
Title: Transplantation of bone marrow mononuclear cells decreases seizure incidence, mitigates neuronal loss and modulates pro-inflammatory cytokine production in epileptic rats.
Authors: Ferro, Zaquer Suzana Munhoz Costa
Souza, Bruno Solano de Freitas
Leal, Marcos Maurício Tosta
Kaneto, Carla Martins
Azevedo, Carine Machado
Silva, Igor Campos da
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
DaCosta, Jaderson Costa
Affilliation: Pontifícia Universidade Católica do Rio Grande do Sul. Instituto de Pesquisas Biomédicas e Instituto do Cérebro. Porto Alegre, RS, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Hospital São Rafael. Serviço de Anatomia Patológica e Citopatologia. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Pontifícia Universidade Católica do Rio Grande do Sul. Instituto de Pesquisas Biomédicas e Instituto do Cérebro. Porto Alegre, RS, Brasil
Abstract: Approximately 30% of patients with mesial temporal lobe epilepsy do not respond to treatment with antiepileptic drugs. We have previously shown that transplantation of mononuclear bone marrow cells (BMC) has an anticonvulsant effect in acute epilepsy. Here, we used pilocarpine to induce epilepsy in rats and studied the effects of BMC injected intravenously either at the onset of seizures or after 10 months of recurrent seizures. BMC effectively decreased seizure frequency and duration. In addition, decreased levels of proinflammatory cytokines (TNF-α, IL-1ß and IL-6) and increased levels of anti-inflammatory cytokine (IL-10) were observed in the brain and serum of BMC-treated rats. Transplants performed at seizure-onset protected against pilocarpine-induced neuronal loss and gliosis and stimulated the proliferation of new neurons in epileptic rats. Our data demonstrate that BMC transplantation has potent therapeutic effects and could be a potential therapy for clinically intractable epilepsies
Keywords: Temporal lobe epilepsy
Bone marrow mononuclear cells
Seizure frequency
Neuronal loss
Cytokines
Pilocarpine
DeCS: Transplante de Medula Óssea
Citocinas/biossíntese
Epilepsia/metabolismo
Leucócitos Mononucleares/transplante
Neurônios/metabolismo
Animais
Transplante de Medula Óssea/métodos
Movimento Celular/fisiologia
Epilepsia/patologia
Incidência
Mediadores da Inflamação/metabolismo
Leucócitos Mononucleares/patologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Neurônios/patologia
Ratos
Ratos Wistar
Issue Date: 2012
Publisher: Elsevier Inc
Citation: COSTA-FERRO, Z. S. M. et al. Transplantation of bone marrow mononuclear cells decreases seizure incidence, mitigates neuronal loss and modulates pro-inflammatory cytokine production in epileptic rats. Neurobiology of Disease, v. 46, n.2, p. 302-313, 2012.
DOI: 10.1016/j.nbd.2011.12.001
ISSN: 1095-953X
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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