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https://www.arca.fiocruz.br/handle/icict/8802
MYCOBACTERIUM TUBERCULOSIS-INDUCED NEUTROPHIL ECTOSOMES DECREASE MACROPHAGE ACTIVATION.
Tuberculosis
Human polymorphonuclear neutrophil
Ectosomes
Mycobacterium tuberculosis
Ativação de Macrófagos/imunologia
Mycobacterium tuberculosis/imunologia
Neutrófilos/imunologia
Tuberculose/imunologia
Comunicação Celular/imunologia
Micropartículas Derivadas de Células/ultraestrutura
Células Cultivadas
Corantes Fluorescentes
Humanos
Macrófagos/imunologia
Microscopia Eletrônica
Neutrófilos/microbiologia
Compostos Orgânicos
Author
Affilliation
Federal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
University College London. Gower Street, London, UK / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
University College London. Gower Street, London, UK / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
Abstract
BACKGROUND: The existence of ectosome-like microvesicles released by neutrophils was proposed a few decades ago. Other studies revealed that the innate immune response during mycobacterial infection is accompanied by an intense migration of neutrophils to the site of infection, which may be important during the acute phase of tuberculosis. We found that the ectosomes derived from infected neutrophils are biologically active and can influence the survival of Mycobacterium tuberculosis within macrophages. METHODS: Mycobacteria were cultured on supplemented Middlebrook-7H9 broth. All strains were grown to the exponential phase and quantitated by serial dilution. Human neutrophils and macrophages were infected with mycobacteria. Ectosomes from neutrophils were isolated post-infection and characterized by transmission electron microscopy and flow cytometry. To determine whether these microvesicles influenced mycobactericidal activity, mycobacteria-infected macrophages were treated with isolated ectosomes. RESULTS: Ectosomes were released from neutrophils infected with mycobacteria. These ectosomes were derived from neutrophil plasma membrane and a small proportion stained with PKH26. These microvesicles, when incubated with infected macrophages, influenced antimycobacterial activity. CONCLUSIONS: This is the first study to demonstrate that ectosomes that are shed from infected neutrophils influence mycobactericidal activity in macrophages in vitro, suggesting that these microvesicles have biological significance. Nevertheless, major gaps in our knowledge of microvesicle biology remain.
Keywords
Cellular immune responseTuberculosis
Human polymorphonuclear neutrophil
Ectosomes
Mycobacterium tuberculosis
DeCS
Micropartículas Derivadas de Células/imunologiaAtivação de Macrófagos/imunologia
Mycobacterium tuberculosis/imunologia
Neutrófilos/imunologia
Tuberculose/imunologia
Comunicação Celular/imunologia
Micropartículas Derivadas de Células/ultraestrutura
Células Cultivadas
Corantes Fluorescentes
Humanos
Macrófagos/imunologia
Microscopia Eletrônica
Neutrófilos/microbiologia
Compostos Orgânicos
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