Author | Luca, Bianca de | pt_BR |
Author | Xavier-Elsas, Pedro Paulo | pt_BR |
Author | Barradas, Mônica | pt_BR |
Author | Luz, Ricardo A. | pt_BR |
Author | Queto, Túlio | pt_BR |
Author | Jones, Carla | pt_BR |
Author | Arruda, Maria Augusta | pt_BR |
Author | Cunha, Thiago Mattar | pt_BR |
Author | Cunha, Fernando Queiroz | pt_BR |
Author | Gaspar-Elsas, Maria Ignez | pt_BR |
Access date | 2014-11-06T17:31:58Z | |
Available date | 2014-11-06T17:31:58Z | |
Document date | 2013 | pt_BR |
Citation | LUCA, Bianca de. et al. Essential roles of PKA, iNOS, CD95/CD95L, and terminal caspases in suppression of eosinopoiesis by PGE2 and other cAMP-elevating agents. ScientificWorld Journal., 2013. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/8752 | |
Language | eng | pt_BR |
Publisher | Hindawi Publishing Corporation | pt_BR |
Rights | open access | pt_BR |
Title | Essential roles of PKA, iNOS, CD95/CD95L, and terminal caspases in suppression of eosinopoiesis by PGE2 and other cAMP-elevating agents | pt_BR |
Type | Article | pt_BR |
DOI | 10.1155/2013/208705 | pt_BR |
Abstract | Up- and downregulation of eosinopoiesis control pulmonary eosinophilia in human asthma. In mice, eosinopoiesis is suppressed
in vitro by prostaglandin E2 (PGE2) and in vivo by diethylcarbamazine, through a proapoptotic mechanism sequentially requiring
inducible NO synthase (iNOS) and the ligand for death receptor CD95 (CD95L).We examined the roles of iNOS, cAMP-mediated
signaling, caspases, and CD95L/CD95 in suppression of eosinopoiesis by PGE2 and other agents signaling through cAMP. Bonemarrow
collected from BALB/c mice, or from iNOS-, CD95-, or CD95L-deficient mutants (and wild-type controls), was cultured
with interleukin-5 (IL-5), alone or associated with PGE2, cAMP-inducing/mimetic agents, caspase inhibitor zVAD-fmk, iNOS
inhibitor aminoguanidine, or combinations thereof, and eosinopoiesis was evaluated at various times. PGE2, added up to 24
hours of culture, dose-dependently suppressed eosinopoiesis, by inducing apoptosis. This effect was (a) paralleled by induction
of iNOS in eosinophils; (b) duplicated by sodium nitroprusside, isoproterenol, and cAMP-inducing/mimetic agents; (c) prevented
by protein kinase A inhibition. NO was produced through iNOS by dibutyryl-cAMP-stimulated bone-marrow. Overall, PGE2 and
isoproterenol shared a requirement for four effector elements (iNOS, CD95L, CD95, and terminal caspases), which together define
a pathway targeted by several soluble up- and downmodulators of eosinopoiesis, including drugs, mediators of inflammation, and
cytokines. | pt_BR |
Affilliation | Fundação Oswalado Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswalado Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Farmacologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Farmacologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswalado Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil. | pt_BR |
DeCS | Eosinofilia Pulmonar | pt_BR |
DeCS | Asma | pt_BR |
DeCS | Medula Óssea | pt_BR |
DeCS | Ratos Mutantes | pt_BR |
DeCS | Eosinófilos | pt_BR |