Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/8713
Title: Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing.
Authors: Oliveira, Susana Carla Pires Sampaio
Monteiro, Juliana Santos de Carvalho
Cangussú, Maria Cristina Teixeira
Santos, Gustavo Miranda Pires
Santos, Marcos André Vannier dos
Santos, Jean Nunes dos
Pinheiro, Antonio Luiz Barbosa
Affilliation: Federal University of Bahia. Center of Biophotonics. School of Dentistry. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonics. School of Dentistry. Salvador, BA, Brasil
Federal University of Bahia. Oral Epidemiology and Public Health. School of Dentistry. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biomorfologia Parasitária. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biomorfologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Laboratory of Surgical Pathology, School of Dentistry. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonics. School of Dentistry. Salvador, BA, Brasil
Abstract: Iron deficiency impairs the formation of hemoglobin, red blood cells, as well the transport of oxygen. The wound healing process involves numerous functions, many of which are dependent on the presence of oxygen. Laser has been shown to improve angiogenesis, increases blood supply, cell proliferation and function. We aimed to study the effect of λ660 nm laser and λ700 nm light-emitting diode (LED) on fibroblastic proliferation on cutaneous wounds on iron-deficient rodents. Induction of iron anemia was carried out by feeding 105 newborn rats with a special iron-free diet. A 1 × 1 cm wound was created on the dorsum of each animal that were randomly distributed into seven groups: I, control anemic; II, anemic no treatment; III, anemic+L; IV, anemic+LED; V, healthy no treatment; VI, healthy+laser; VII, healthy+LED (n=15 each). Phototherapy was carried out using either a diode laser (λ660 nm, 40 mW, 10 J/cm(2)) or a prototype LED device (λ700 ± 20 nm, 15 mW, 10 J/cm(2)). Treatment started immediately after surgery and was repeated at 48-h interval during 7, 14, and 21 days. After animal death, specimens were taken, routinely processed, cut, stained with hematoxylin-eosin, and underwent histological analysis and fibroblast counting. Significant difference between healthy and anemic subjects on regards the number of fibroblast between treatments was seen (p<0.008, p<0.001). On healthy animals, significant higher count was seen when laser was used (p<0.008). Anemic subjects irradiated with LED showed significantly higher count (p<0.001). It is concluded that the use of LED light caused a significant positive biomodulation of fibroblastic proliferation on anemic animals and laser was more effective on increasing proliferation on non-anemics.
DeCS: Terapia a Laser de Baixa Intensidade
Fototerapia
Pele/lesões
Pele/efeitos de radiação
Cicatrização/efeitos de radiação
Anemia Ferropriva/patologia
Anemia Ferropriva/radioterapia
Animais
Animais Recém-Nascidos
Proliferação de Células/efeitos de radiação
Modelos Animais de Doenças
Fibroblastos/patologia
Luz
Masculino
Ratos
Pele/patologia
Issue Date: 2013
Publisher: Springer
Citation: OLIVEIRA, S. C. P. S. et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Medical Sciences, v. 28, n. 3, p. 799-806, 2013.
DOI: 10.1007/s10103-012-1161-9
ISSN: 1435-604X
Copyright: open access
Appears in Collections:CPqGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Oliveira SCPS Effect of laser....pdf582.5 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.