Author | Bomfim, Diogo S. | |
Author | Ferraz, Rosana Paula Cruz | |
Author | Carvalho, Nanashara Coelho de | |
Author | Soares, Milena Botelho Pereira | |
Author | Pinheiro, Maria Lúcia Bélem | |
Author | Costa, Emmanoel Vilaça | |
Author | Bezerra, Daniel Pereira | |
Access date | 2014-10-09T14:02:22Z | |
Available date | 2014-10-09T14:02:22Z | |
Document date | 2013 | |
Citation | BOMFIM, Diogo D. et al. Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells. Basic & Clinical Pharmacology & Toxicology, v. 113, n. 5, p. 300-306, 2013. | pt_BR |
ISSN | 1742-7843 | |
URI | https://www.arca.fiocruz.br/handle/icict/8565 | |
Language | eng | pt_BR |
Publisher | John Wiley & Sons Ltd | pt_BR |
Rights | open access | pt_BR |
Title | Eudesmol isomers induce caspase-mediated apoptosis in human hepatocellular carcinoma HepG2 cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/bcpt.12097 | |
Abstract | Eudesmols are naturally occurring sesquiterpenoid alcohols that present cytotoxic effect to cancer cells. Herein, all eudesmol isomers displayed cytotoxicity to different tumour cell lines. a-Eudesmol showed IC50 values ranging from 5.38 1.10 to 10.60 1.33 lg/mL for B16-F10 and K562 cell lines, b-eudesmol showed IC50 values ranging from 16.51 1.21 to 24.57 2.75 lg/mL for B16-F10 and HepG2 cell lines, and c-eudesmol showed IC50 values ranging from 8.86 1.27 to 15.15 1.06 lg/mL for B16-F10 and K562 cell lines, respectively. In addition, in this work, we studied the mechanisms of cytotoxic action of eudesmol isomers (a-, b- and c-eudesmol) in human hepatocellular carcinoma HepG2 cells. After 24-hr incubation, HepG2 cells treated with eudesmol isomers presented typical hallmarks of apoptosis, as observed by morphological analysis in cells stained with haematoxylin–eosin and acridine orange/ethidium bromide. None of eudesmol isomers caused membrane disruption at any concentration tested. Moreover, eudesmol isomers induced loss of mitochondrial membrane potential and an increase in caspase-3 activation in HepG2 cells, suggesting the induction of caspase-mediated apoptotic cell death. In conclusion, the eudesmol isomers herein investigated are able to reduce cell proliferation and to induce tumour cell death by caspase-mediated apoptosis pathways. | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Crist ovão. Aracaju, SE, Brasil. | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Crist ovão. Aracaju, SE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brasil. | pt_BR |
Affilliation | Federal University of Sergipe. Department of Chemistry. São Cristovão. Aracaju, SE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | Eudesmol Isomers | pt_BR |
Subject | Human Hepatocellular | pt_BR |
Subject | Carcinoma HepG2 Cells | pt_BR |
DeCS | Apoptose/efeitos de drogas | pt_BR |
DeCS | Sesquiterpenos de Eudesmano/farmacologia | pt_BR |
DeCS | Carcinoma Hepatocelular/metabolismo | pt_BR |
DeCS | Carcinoma Hepatocelular/patologia | pt_BR |
DeCS | Caspase 3/genética | pt_BR |
DeCS | Caspase 3/metabolismo | pt_BR |
DeCS | Linhagem Celular Tumoral | pt_BR |
DeCS | Membrana Celular/efeitos de drogas | pt_BR |
DeCS | Proliferação de Células/efeitos de drogas | pt_BR |
DeCS | Células Hep G2 | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Concentração Inibidora 50 | pt_BR |
DeCS | Células K562 | pt_BR |
DeCS | Potencial da Membrana Mitocondrial/efeitos de drogas | pt_BR |