Author | Coutinho Junior, Raimundo | |
Author | Grassi, Maria Fernanda Rios | |
Author | Korngold, Ana Beatriz | |
Author | Gallazzi, Viviana Nilla Olavarria | |
Author | Castro Filho, Bernardo Galvão | |
Author | Mascarenhas, Rita Elizabeth Moreira | |
Access date | 2014-09-26T13:27:12Z | |
Available date | 2014-09-26T13:27:12Z | |
Document date | 2014 | |
Citation | COUTINHO JUNIOR, R. et al. Human T lymphotropic virus type 1 (HTLV-1) proviral load induces activation of T-lymphocytes in asymptomatic carriers. BMC Infectious Diseases, v. 14, p. 53, 2014. | pt_BR |
ISSN | 1471-2334 | |
URI | https://www.arca.fiocruz.br/handle/icict/8454 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Title | Human T lymphotropic virus type 1 (HTLV-1) proviral load induces activation of T-lymphocytes in asymptomatic carriers. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1471-2334-14-453 | |
Abstract | Background: High HTLV-1 proviral load (PVL) is mainly found in infected individuals with HTLV-1-associated
myelopathy/tropical spastic paraparesis (HAM/TSP). However one third of asymptomatic carriers may have high PVL.
This study aimed to evaluate the impact of PVL in the activation of T lymphocytes of asymptomatic individuals
infected with HTLV-1.
Methods: Membrane activation markers (CD25+, CD28+, CD45RO+, CD69+, CD62L+, HLA-DR+), FoxP3+ and intracellular
IFN-γ expression were evaluated on both CD4+ and CD8+ T-lymphocytes from asymptomatic carriers with PVL ≥
and < 1% of infected cells, using flow cytometry. HTLV-1 proviral load was determined using real-time PCR.
Results: Asymptomatic carriers with PVL ≥ 1% presented a higher frequency of CD4+CD25+CD45RO+ (13.2% vs. 4%,
p = 0.02), CD4+HLA-DR+ (18% vs. 8.3%, p = 0.01) and CD4+IFN-γ+ (4.5%; 1%, p = 0.01) T-cells, than healthy donors.
HTLV-1 PVL was directly correlated with the proportion of CD4+CD25+CD45RO+ T-cells (R = 0.7, p = 0.003). Moreover, a
significant increase in the proportion of CD4 + FoxP3+ T-cells was observed in HTLV-1-infected individuals, compared
to healthy donors.
Conclusion: HTLV-1 PVL is associated with activation of both CD4+ and CD8+ T-lymphocytes in asymptomatic
individuals. Prospective studies should be conducted to evaluate whether asymptomatic individuals with higher PVL
and high immune activation are more prone to developing HTLV-1-associated diseases | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Bahiana School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Salvador, BA, Brasil | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | Proviral load | pt_BR |
Subject | Asymptomatic | pt_BR |
Subject | Activation | pt_BR |