Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Tomlinson, Stephen | |
Author | Nussenzweig, Victor | |
Access date | 2014-09-10T12:37:51Z | |
Available date | 2014-09-10T12:37:51Z | |
Document date | 1993 | |
Citation | PONTES-DE-CARVALHO, L. C. et al. Trypanosoma rangeli sialidase lacks trans-sialidase activity. Molecular Biochemical Parasitology, v. 62, n. 1, p. 19-25, 1993. | pt_BR |
ISSN | 0166-6851 | |
URI | https://www.arca.fiocruz.br/handle/icict/8339 | |
Language | eng | pt_BR |
Publisher | Elsevier Science Publishers B.V | pt_BR |
Rights | open access | pt_BR |
Title | Trypanosoma rangeli sialidase lacks trans-sialidase activity. | pt_BR |
Type | Article | pt_BR |
Abstract | Extracts and tissue culture supernatants of axenic forms of T. rangeli were assayed for the presence of sialidase and trans-sialidase activities. Using sialyl(alpha 2-3)lactose, sialyl(alpha 2-6)lactose, poly(alpha 2-8)N-acetylneuraminic acid, fetuin and 4-methylumbelliferyl-N-acetylneuraminic acid as sialic acid donors, and lactose as a sialic acid acceptor, no trans-sialidase activity was detected. Nevertheless, T. rangeli lysates and culture supernatants contain a sialidase that hydrolyzes sialyl(alpha 2-3)lactose, and much less efficiently sialyl(alpha 2-6)lactose, but not poly(alpha 2-8)N-acetylneuraminic acid. T. cruzi trans-sialidase hydrolyzed only sialyl(alpha 2-3)lactose under the same conditions. The T. rangeli and the T. cruzi enzymes differ antigenically and in their pH optimum for hydrolase activity. | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. Department of Pathology and Kaplan Cancer Center. New York, NY / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. Department of Pathology and Kaplan Cancer Center. New York, NY | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. Department of Pathology and Kaplan Cancer Center. New York, NY | pt_BR |
Subject | Trypanosoma rangeli | pt_BR |
Subject | Sialidase | pt_BR |
Subject | Neuraminidase | pt_BR |
Subject | Trans-sialidase | pt_BR |
DeCS | Glicoproteínas | pt_BR |
DeCS | Neuraminidase/metabolismo | pt_BR |
DeCS | Trypanosoma/enzimologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Antígenos de Protozoários | pt_BR |
DeCS | Concentração de Íons de Hidrogênio | pt_BR |
DeCS | Imunoquímica | pt_BR |
DeCS | Neuraminidase/imunologia | pt_BR |
DeCS | Ácidos Siálicos/metabolismo | pt_BR |
DeCS | Especificidade da Espécie | pt_BR |
DeCS | Especificidade por Substrato | pt_BR |
DeCS | Trypanosoma/imunologia | pt_BR |
DeCS | Trypanosoma brucei brucei/enzimologia | pt_BR |
DeCS | Trypanosoma cruzi/enzimologia | pt_BR |