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HIGH FREQUENCY OF RECOMBINANT GENOMES IN HIV TYPE 1 SAMPLES FROM BRAZILIAN SOUTHEASTERN AND SOUTHERN REGIONS.
HIV-1/classificação
HIV-1/genética
Recombinação Genética
Sequência de Bases
Brasil
Frequência do Gene
Proteína gp120 do Envelope de HIV/genética
Proteína gp41 do Envelope de HIV/genética
Dados de Sequência Molecular
Filogenia
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Immunology. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Immunology. Rio de Janeiro, RJ, Brasil
Public Healthy Secretariat of RS State. Porto Alegre, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Immunology. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Immunology. Rio de Janeiro, RJ, Brasil
Public Healthy Secretariat of RS State. Porto Alegre, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Immunology. Rio de Janeiro, RJ, Brasil
Abstract
We describe the genetic variability of HIV-1 subtypes and recombinant genomes in samples from southeastern
and southern Brazilian regions. Phylogenetic analysis of a subset of 34 samples (8F, 7B, 7C, 2D, 1A, and
9 B0 variant) based on the DNA sequencing of the env gp120 and gp41, gag p17, and nef regions confirmed
the presence of nine (26.5%) potentially HIV-1 recombinant genomes. From the eight C2–V3 gp120 subtype
F samples, only two seem to be pure F. One of the samples, classified as B0 in the C2–V3 gp120 and as B in
gp41 had the gag and nef regions clustering with subtype C. Two of seven C2–V3 subtype C samples presented
distinct recombinant patterns as Bgag/Cenv/Bnef and Bgag/Cenv/Cnef. Putative recombinant breakpoints
were obtained for three samples presenting discordant subtypes (F/B) between gp120 and gp41 env
fragments showing that similar breakpoints could be observed between two unlinked samples (95BRRJ014
and 96BRRJ101). A higher degree of polymorphism was verified in the analysis of a subtype A sample
(98BRRS058) in the C2–V3/gp41 env fragment. The intrasubtype C distance was found to be lower than that
found for the other subtypes for all genomic regions. These data confirm that distinct HIV-1 subtypes and
recombinant forms are actively participating in the Brazilian AIDS epidemic, and that the subtype C was introduced
more recently into southern Brazil.
DeCS
Genoma ViralHIV-1/classificação
HIV-1/genética
Recombinação Genética
Sequência de Bases
Brasil
Frequência do Gene
Proteína gp120 do Envelope de HIV/genética
Proteína gp41 do Envelope de HIV/genética
Dados de Sequência Molecular
Filogenia
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