Author | Pinheiro, Melina de Barros | |
Author | Antonelli, Lis Ribeiro do Valle | |
Author | Avelar, Renato Sathler | |
Author | Avelar, Danielle Marquete Vitelli | |
Author | Miranda, Silvana Spindola de | |
Author | Guimarães, Tânia Mara Pinto Dabés | |
Author | Carvalho, Andréa Teixeira de | |
Author | Martins Filho, Olindo Assis | |
Author | Toledo, Vicente de Paulo Coelho Peixoto de | |
Access date | 2014-06-18T17:38:47Z | |
Available date | 2014-06-18T17:38:47Z | |
Document date | 2012 | |
Citation | PINHEIRO, Melina de Barros et al. CD4-CD8-αβ and γδ T cells display inflammatory and regulatory potentials during human tuberculosis. Plos One. 2012, vol.7, pp. 50923 | pt_BR |
ISSN | 1932-6203 | |
URI | https://www.arca.fiocruz.br/handle/icict/7829 | |
Language | eng | pt_BR |
Publisher | Public Library of Science one | pt_BR |
Rights | open access | pt_BR |
Title | CD4-CD8-αβ and γδ T cells display inflammatory and regulatory potentials during human tuberculosis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0050923 | |
Abstract | T-cells play an important role controlling immunity against pathogens and therefore influence the outcome of human diseases. Although most T-lymphocytes co-express either CD4 or CD8, a smaller T-cell subset found the in the human peripheral blood that expresses the αβ or γδ T-cell-receptor (TCR) lacks the CD4 and CD8 co-receptors. These double negative (DN) T-cells have been shown to display important immunological functions in human diseases. To better understand the role of DN T-cells in human Mycobacterium tuberculosis, we have characterized their frequency, activation and cytokine profile in a well-defined group of tuberculosis patients, categorized as severe and non-severe based on their clinical status. Our data showed that whereas high frequency of αβ DN T-cells observed in M. tuberculosis-infected patients are associated with disease severity, decreased proportion of γδ DN T-cells are found in patients with severe tuberculosis. Together with activation of CD4+ and CD8+ T-cells, higher frequencies of both αβ and γδ DN T-cells from tuberculosis patients also express the chronic activation marker HLA-DR. However, the expression of CD69, an early activation marker, is selectively observed in DN T-cells. Interestingly, while αβ and γδ DN T-cells from patients with non-severe tuberculosis display a pro-inflammatory cytokine profile, characterized by enhanced IFN-γ, the γδ DN T-cells from patients with severe disease express a modulatory profile exemplified by enhanced interleukin-10 production. Overall, our findings suggest that αβ and γδ DN T-cell present disparate immunoregulatory potentials and seems to contribute to the development/maintenance of distinct clinical aspects of TB, as part of the complex immunological network triggered by the TB infection. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Clínica Médica. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Mycobacterium tuberculosis | pt_BR |
Subject | T-cells | pt_BR |