Author | Pereira, Valéria Rêgo Alves | |
Author | Reis, Luiza de Campos | |
Author | Souza, Marina de Assis | |
Author | Oliveira, Andresa Pereira de | |
Author | Brito, Maria Edileuza Felinto de | |
Author | Lage, Paula Souza | |
Author | Andrade, Mariléia Chaves de | |
Author | Rocha, Roberta Dias Rodrigues | |
Author | Martins Filho, Olindo Assis | |
Access date | 2014-06-17T14:43:21Z | |
Available date | 2014-06-17T14:43:21Z | |
Document date | 2012 | |
Citation | PEREIRA, Valéria Rêgo Alves et al. Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis. Diagnostic Microbiology and Infectious Disease. 2012, vol.74, pp. 292-298 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/7826 | |
Sponsorship | State of Pernambuco Research Foundation (FACEPE), by the Brazilian National Research Council (CNPq), and by the Oswaldo Cruz Foundation (FIOCRUZ). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier B.V. | pt_BR |
Rights | open access | pt_BR |
Title | Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.diagmicrobio.2012.06.025 | |
Abstract | This study aims to investigate a flow cytometry performance–based methodology to detect anti-live (FC-ALPA-IgG) and anti-fixed (FC-AFPA-IgG) Leishmania (Viannia) braziliensis promastigote IgG as a means to monitor post-therapeutic cure of patients with localized cutaneous leishmaniasis (LCL). Serum samples from 30 LCL patients infected with L. (V.) braziliensis were assayed, comparing the IgG reactivity before and after specific treatment with pentavalent antimonial. Reactivities were reported as the percentage of positive fluorescent parasites (PPFP), using a PPFP of 60% as a cut-off value. In the serum dilution of 1:1024, the positive percentage of LCL serum sample for FC-ALPA-IgG and FC-AFPA-IgG was 86% and 90%, respectively, before treatment. Analysis of ∆PPFP that represents the difference between PPFP after and before treatment appeared as a new approach to monitor post-therapeutic IgG reactivity in LCL. Our data support the perspective of using FC-ALPA and FC-AFPA as a useful serologic tool for diagnosis and for post-therapeutic follow-up of LCL patients. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Universidade Estadual de Montes Claros. Montes Claros, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou.Belo Horizonte, MG, Brazil/Centro Universitário Newton Paiva. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou.Belo Horizonte, MG, Brazil | pt_BR |
Subject | Localized cutaneous leishmaniasis | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | FC-ALPA | pt_BR |
Subject | FC-AFPA | pt_BR |
Subject | IgG | pt_BR |
Subject | L. (V.) braziliensis | pt_BR |