Author | Leoratti, Fabiana Maria de Souza | |
Author | Trevelin, Silvia Cellone | |
Author | Cunha, Fernando Queiroz | |
Author | Rocha, Bruno Coelho | |
Author | Costa, Pedro Augusto Carvalho | |
Author | Gravina, Humberto Doriguêtto | |
Author | Tada, Mauro Shugiro | |
Author | Pereira, Dhelio Batista | |
Author | Golenbock, Douglas Taylor | |
Author | Antonelli, Lis Ribeiro do Valle | |
Author | Gazzinelli, Ricardo Tostes | |
Access date | 2014-05-22T12:55:03Z | |
Available date | 2014-05-22T12:55:03Z | |
Document date | 2012 | |
Citation | LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1710 | pt_BR |
ISSN | 1935-2735 | |
URI | https://www.arca.fiocruz.br/handle/icict/7635 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Neutrophil paralysis in Plasmodium vivax malaria | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0001710 | |
Abstract | Background
The activation of innate immune responses by Plasmodium vivax results in activation of effector cells and an excessive production of pro-inflammatory cytokines that may culminate in deleterious effects. Here, we examined the activation and function of neutrophils during acute episodes of malaria.
Materials and Methods
Blood samples were collected from P. vivax-infected patients at admission (day 0) and 30–45 days after treatment with chloroquine and primaquine. Expression of activation markers and cytokine levels produced by highly purified monocytes and neutrophils were measured by the Cytometric Bead Assay. Phagocytic activity, superoxide production, chemotaxis and the presence of G protein-coupled receptor (GRK2) were also evaluated in neutrophils from malaria patients.
Principal Findings
Both monocytes and neutrophils from P. vivax-infected patients were highly activated. While monocytes were found to be the main source of cytokines in response to TLR ligands, neutrophils showed enhanced phagocytic activity and superoxide production. Interestingly, neutrophils from the malaria patients expressed high levels of GRK2, low levels of CXCR2, and displayed impaired chemotaxis towards IL-8 (CXCL8).
Conclusion
Activated neutrophils from malaria patients are a poor source of pro-inflammatory cytokines and display reduced chemotactic activity, suggesting a possible mechanism for an enhanced susceptibility to secondary bacterial infection during malaria. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/ Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/ Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brazil | pt_BR |
Affilliation | Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of America | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of America/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Plasmodium vivax | pt_BR |
Subject | Malaria | pt_BR |
Subject | Immune response | pt_BR |
Subject | Neutrophils | pt_BR |
Subject | G protein-coupled receptor kinases | pt_BR |
Subject | Chemotaxis | pt_BR |