Author | Miranda, Anderson Fontes Suzart | |
Author | Santos, Gustavo Miranda Pires | |
Author | Oliveira, Susana Carla Pires Sampaio | |
Author | Monteiro, Juliana Santos de Carvalho | |
Author | Sampaio, Fernando José Pires | |
Author | Gomes Júnior, Rafael Araújo | |
Author | Brugnera Junior, Aldo | |
Author | Gesteira, Maria de Fatima Malvar | |
Author | Zanin, Fátima A. A | |
Author | Pinheiro, Antonio Luiz Barbosa | |
Author | Santos, Marcos André Vannier dos | |
Access date | 2014-05-09T17:40:17Z | |
Available date | 2014-05-09T17:40:17Z | |
Document date | 2014 | |
Citation | MIRANDA, A. F. et al. Phenothiazinium dyes in association with diode red laser against. Progress in Biomedical Optics and Imaging Proceedings of SPIE, v. 893212, p. 1-6, 2014. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/7597 | |
Language | eng | pt_BR |
Publisher | SPIE | pt_BR |
Rights | open access | pt_BR |
Title | Phenothiazinium dyes in association with diode red laser against | pt_BR |
Type | Article | pt_BR |
DOI | 10.1117/12.2038466 | |
Abstract | In Brazil solar incidence is high and continuous throughout the year. Body exposure to sunlight may be a key point in the
rates of individuals affected by melanoma and other types of skin cancer in many countries. Brazil already occupies the
15th place in the ranking of melanoma cases and the limitations presented by drugs used in the therapy of this cancer,
new approaches are being used in an attempt to decrease the mortality of this malignancy. The aim of this study was to
evaluate the effects of phenothiazinium dyes (PD) associated with laser light on murine melanoma (B16F10) in vitro by
measuring cell growth using colorimetric assay before and after photodynamic therapy. We used a diode laser (λ660nm,
2.4 J/cm², 40 mW, 60 s, CW) associated with PD at 12.5 μg/mL, time pre-irradiation of 30 minutes). The following
groups were tested: control (LF-), PD (L-F+), Laser (L+F-), Laser + PD (L+F+). The results showed a significant
reduction in cell growth in the group treated by the photodynamic therapy compared to the control at 24 and 48 h (p <
0.001). Were showing at 30 min PD has a dose-dependent response on B16F10 cells, but at 24 h did not demonstrated
this response. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics, São Carlos, SP, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics, São Carlos, SP, Brasil / Department of Biology. Estate University of Feira de Santana. Feira de Santana, BA, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics, São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics, São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Affilliation | Center of Biophotonic. Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics, São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Subject | Light | pt_BR |
Subject | PDT | pt_BR |
Subject | Melanoma | pt_BR |
Subject | Laser | pt_BR |