Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/7403
Title: Changes in amounts of total salivary gland proteins of Lutzomyia longipallpis (Diptera: Psychodidae) according to age and diet
Authors: Prates, Deboraci Brito
Santos, L. D.
Miranda, José Carlos
Souza, A. P. A.
Palma, Mário Sérgio
Barral Netto, Manoel
Barral, Aldina Maria Prado
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Universidade Estadual Paulista de Rio Claro. Instituto de Biociências de Rio Claro. Departamento de Biologia. Laboratório de Biologia Estrutural e Zooquímica. Rio Claro, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Instituto de Investigação em Imunologia iii. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto de Investigação em Imunologia iii. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto de Investigação em Imunologia iii. Salvador, BA, Brasil
Abstract: Saliva plays important roles in facilitation of a bloodmeal, lubrication of mouthparts, and parasite transmission for some vector insects. Salivary composition changes during the lifetime of an insect, and differences in the salivary proÞle may inßuence its functions. In this report, the amount and proÞle of salivary gland protein of the American visceral leishmaniasis vector Lutzomyia longipalpis (Lutz & Neiva, 1912) were analyzed at different times of insect development and diet. Protein content from unfed female sand ßies increased signiÞcantly with age, and a signiÞcant difference was observed in sugar-fed females during the Þrst 10 d of adult life. Salivary protein content sharply decreased 1 d after blood feeding, with gradual increase in concentration the following days. SDSpolyacrylamide gel electrophoresis analysis revealed that most polypeptides present in the saliva of sugar-fed also were present in the saliva of blood-fed females. Understanding changes in sand ßyÕs saliva contents at distinct days after emergence and the inßuence of a bloodmeal in this aspect may reveal the role played by saliva during leishmaniasis transmission.
Keywords: Lutzomyia longipalpis
Salivary gland
Saliva
SDS-polyacrylamide gel electrophoresis
DeCS: Envelhecimento/fisiologia
Dieta
Proteínas de Insetos/metabolismo
Psychodidae/metabolismo
Proteínas e Peptídeos Salivares/metabolismo
Animais
Feminino
Regulação da Expressão Gênica
Issue Date: 2008
Publisher: Entomological Society of America
Citation: PRATES, D. B. et al. Changes in amounts of total salivary gland proteins of Lutzomyia longipallpis (Diptera: Psychodidae) according to age and diet. Journal of Medical Entomology, v. 45, n. 3, p. 409-413, 2008.
ISSN: 0022-2585
Copyright: open access
Appears in Collections:CPqGM - Artigos de Periódicos

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