Author | Cajado, Cyntia | |
Author | Cerqueira, Bruno Antonio Veloso | |
Author | Couto, Fábio David | |
Author | Santos, Wendell Vilas Boas | |
Author | Dorea, M. J | |
Author | Lyra, Isa Menezes | |
Author | Barbosa, Cynara Gomes | |
Author | Reis, Mitermayer Galvão dos | |
Author | Gonçalves, Marilda de Souza | |
Access date | 2014-02-10T16:48:34Z | |
Available date | 2014-02-10T16:48:34Z | |
Document date | 2011 | |
Citation | CAJADO, C. et al. TNF-alpha and IL-8: serum levels and gene polymorphisms (-308G>A and -251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia. Cytokine, v. 56, n. 2, p. 312-317, 2011. | pt_BR |
ISSN | 1043-4666 | |
URI | https://www.arca.fiocruz.br/handle/icict/7309 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | TNF-alpha and IL-8: serum levels and gene polymorphisms (-308G>A and -251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.cyto.2011.07.002 | |
Abstract | Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present
study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) 308G>A and
Interleukin 8 (IL-8) 251A>T gene polymorphisms, medical history and classical biomarkers in children
with steady-state SCA. In total, 210 SCA patients aged 2–21 years and 200 healthy controls were studied.
Gene polymorphisms, betaS-globin haplotypes and a 3.7-kb deletion in alpha2-thalassemia (a2-thal3.7 kb)
were investigated by PCR/RFLP analysis, and cytokine levels were determined by ELISA. Splenomegaly
(p = .032) was more prevalent among children younger than 5 years of age. The A allele of the TNF-alpha
308G>A gene polymorphism and the presence of a2-thal3.7 kb were associated with an increase risk of
splenic sequestration events (p = .001; p = .046), while the T allele of the IL-8 251A>T gene polymorphism
was considered to be a protective factor for splenomegaly events (p = .032). Moreover, the A allele
of the TNF-alpha 308G>A gene polymorphism was associated with high TNF-alpha levels (p = .021), and
the hemoglobin F and hemoglobin S haplotypes were correlated with serum levels of IL-8. The logistic
regression analysis showed significant effects of the TNF-alpha and IL-8 gene polymorphisms, betaS-globin
gene haplotypes and a2-thal3.7 kb on the occurrence of splenic sequestration events. Our study emphasizes
that the identification of new genetic and immunological biomarkers and their associations with
classical markers is an important strategy to elucidate the underlying causes of different SCA phenotypes
and their effects on patient outcome | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Estado da Bahia. HEMOBA. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil | pt_BR |
Subject | Sickle cell anemia | pt_BR |
Subject | TNF-alpha | pt_BR |
Subject | IL-8 | pt_BR |
Subject | betaS-globin Gene haplotypes | pt_BR |
Subject | alpha2-Thalassemia | pt_BR |
DeCS | Anemia Falciforme/sangue | pt_BR |
DeCS | Marcadores Biológicos/sangue | pt_BR |
DeCS | Interleucina-8/sangue | pt_BR |
DeCS | Anamnese | pt_BR |
DeCS | Polimorfismo Genético | pt_BR |
DeCS | Fator de Necrose Tumoral alfa/sangue | pt_BR |
DeCS | Adolescente | pt_BR |
DeCS | Adulto | pt_BR |
DeCS | Alelos | pt_BR |
DeCS | Anemia Falciforme/genética | pt_BR |
DeCS | Estudos de Casos e Controles | pt_BR |
DeCS | Criança | pt_BR |
DeCS | Pré-Escolar | pt_BR |
DeCS | Estudos Transversais | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
DeCS | Haplotipos | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Interleucina-8/genética | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Polimorfismo de Fragmento de Restrição | pt_BR |
DeCS | Fator de Necrose Tumoral alfa/genética | pt_BR |