Author | Cruz, Ebenézer de Mello | |
Author | Silva, Edson Roberto da | |
Author | Maquiaveli, Claudia do Carmo | |
Author | Alves, Eliomara Sousa Sobral | |
Author | Lucon Junior, João Francisco | |
Author | Reis, Matheus Balduino Gonçalves dos | |
Author | Toledo, Cleyton Eduardo Mendes de | |
Author | Cruz, Frederico Guaré | |
Author | Santos, Marcos André Vannier dos | |
Access date | 2013-10-23T17:55:30Z | |
Available date | 2013-10-23T17:55:30Z | |
Document date | 2013 | |
Citation | CRUZ, E. de M. et al. Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement. Phytochemistry, v. 89, p. 71-77, 2013. | pt_BR |
ISSN | 1873-3700 | |
URI | https://www.arca.fiocruz.br/handle/icict/7191 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement | pt_BR |
Type | Article | pt_BR |
Abstract | The plant Cecropia pachystachya Trécul is widely used in Brazilian ethnomedicine to treat hypertension, asthma, and diabetes. Arginase is an enzyme with levels that are elevated in these disorders, and it is central to Leishmania polyamine biosynthesis. The aims of this study were to evaluate antileishmanial activity and inhibition of the arginase enzyme by C. pachystachya extracts, and to study changes in cellular organization using electron microscopy. The ethanol extract of C. pachystachya was tested on Leishmania (Leishmania) amazonensis promastigote survival/proliferation and arginase activity in vitro. Qualitative ultrastructural analysis was also used to observe changes in cell organization. The major bioactive molecules of the ethanol extract were characterized using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The ethyl acetate fraction of the ethanol extract diminished promastigote axenic growth/survival, inhibited arginase activity, and altered a mitochondrial kinetoplast DNA (K-DNA) array. The bioactive compounds of C. pachystachya were characterized as glucoside flavonoids. Orientin (9) (luteolin-8-C-glucoside) was the main component of the methanol-soluble ethyl acetate fraction obtained from the ethanol extract and is an arginase inhibitor (IC50 15.9 µM). The ethyl acetate fraction was not cytotoxic to splenocytes at a concentration of 200 µg/mL. In conclusion, C. pachystachya contains bioactive compounds that reduce the growth of L. (L.) amazonensis promastigotes, altering mitochondrial K-DNA arrangement and inhibiting arginase. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária. São Paulo, SP, BRasil | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Fisiologia. Ribeirão Preto, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Pirassununga, SP, Brazil | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária. São Paulo, SP, BRasil | pt_BR |
Affilliation | Curso de Farmácia. Unidade de Ensino Superior Ingá. Maringa, SP, Brazil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Química. Departamento de Química Orgânica. Salvador, BA, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
Subject | Cecropia pachystachya | pt_BR |
Subject | Cecropiaceae | pt_BR |
Subject | Arginase | pt_BR |
Subject | Polyamines | pt_BR |
Subject | Oxidative stress | pt_BR |
Subject | Kinetoplast | pt_BR |
Subject | Ultrastructure | pt_BR |
Subject | Leishmania | pt_BR |
DeCS | Antiprotozoários/farmacologia | pt_BR |
DeCS | Arginase/antagonistas & inibidores | pt_BR |
DeCS | Cecropia (Planta)/química | pt_BR |
DeCS | DNA Mitocondrial/genética | pt_BR |
DeCS | Inibidores Enzimáticos/farmacologia | pt_BR |
DeCS | Flavonoides/farmacologia | pt_BR |
DeCS | Leishmania/efeitos de drogas | pt_BR |
DeCS | Acetatos/química | pt_BR |
DeCS | Etanol/química | pt_BR |
DeCS | Glucosídeos/farmacologia | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Leishmania | pt_BR |
DeCS | Metanol/química | pt_BR |
DeCS | Solubilidade | pt_BR |