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https://www.arca.fiocruz.br/handle/icict/63820
CAPSULAR POLYSACCHARIDE PRODUCTION BY NEISSERIA MENINGITIDIS SEROGROUP C: OPTIMIZATION OF PROCESS VARIABLES USING RESPONSE SURFACE METHODOLOGY
Capsular polysaccharide
Face-centered central composite design
Response surface methodology
Process variables optimization
Affilliation
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro.
Fundação Oswaldo Cruz. Instituto de tecnologia em imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa em Engenharia. Programa de Engenharia Química. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa em Engenharia. Programa de Engenharia Química. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de tecnologia em imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa em Engenharia. Programa de Engenharia Química. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa em Engenharia. Programa de Engenharia Química. Rio de Janeiro, RJ, Brasil.
Abstract
The production of capsular polysaccharide by Neisseria meningitidis serogroup C in Frantz medium cultivations was studied. Influence of process variables such as temperature, pH and agitation speed on the polysaccharide production were investigated by means of a three-factor facecentered central composite design. An optimal set of those process variables values were calculated using response surface methodology, and results showed that optimal values were: temperature equal to 37 8C, pH controlled at 7.0 and agitation speed maintained at 1300 rpm, which correspond to a oxygen global mass transfer coefficient (kLa) of 36 h 1. The capsular polysaccharide concentration achieved under these conditions was equal to 155 4 mg/L.
Keywords
Neisseria meningitidisCapsular polysaccharide
Face-centered central composite design
Response surface methodology
Process variables optimization
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