Author | Rabello, Vanessa Brito de Souza | |
Author | Teixeira, Marcus de Melo | |
Author | Meyer, Wieland | |
Author | Irinyi, Laszlo | |
Author | Xavier, Melissa Orzechowski | |
Author | Poester, Vanice Rodrigues | |
Author | Brunelli, José Guillermo Pereira | |
Author | Almeida-Silva, Fernando | |
Author | Bernardes-Engemann, Andrea Reis | |
Author | Gremião, Isabella Dib Ferreira | |
Author | Angelo, Débora Ferreira dos Santos | |
Author | Clementino, Inácio José | |
Author | Almeida-Paes, Rodrigo | |
Author | Zancopé-Oliveira, Rosely Maria | |
Access date | 2024-03-13T00:11:28Z | |
Available date | 2024-03-13T00:11:28Z | |
Document date | 2024 | |
Citation | RABELLO, Vanessa Brito de Souza et al. Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis. Fungal Genetics and Biology, v. 170, p. 1-7, Feb. 2024. | en_US |
ISSN | 1087-1845 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/63030 | |
Sponsorship | This research was partially funded by Conselho Nacional de Desen volvimento Científico e Tecnologico ´ (CNPq), CHAMADA CNPq/MCTI/FNDCT N◦ 18/2021 – UNIVERSAL - grant number 405653/2021-2. R.M. Z-O was also supported in part by Conselho Nacional de Desenvolvi mento Científico e Tecnologico ´ (CNPq), grant numbers 308315/021-9 and 316067/2021-0, Fundaçao ˜ Carlos Chagas Filho de Amparo a ` Pes quisa do Estado do Rio de Janeiro (FAPERJ), grant number E-26/200.381/2023. | en_US |
Language | eng | en_US |
Publisher | Elsevier | en_US |
Rights | restricted access | en_US |
Title | Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis | en_US |
Type | Article | en_US |
DOI | 10.1016/j.fgb.2023.103845 | |
Abstract | Sporotrichosis is a subcutaneous mycosis caused by pathogenic Sporothrix species. Among them, Sporothrix brasiliensis is the main species associated with endemic regions in South America, especially Brazil. It is highly virulent and can be spread through zoonotic transmission. Molecular epidemiological surveys are needed to determine the extent of genetic variation, to investigate outbreaks, and to identify genotypes associated with antifungal resistance and susceptibility. This study investigated the sequence variation of different constitutive genes and established a novel multilocus sequence typing (MLST) scheme for S. brasiliensis. Specific primers were designed for 16 genes using Primer-BLAST software based on the genome sequences of three S. brasiliensis strains (ATCC MYA-4823, A001 and A005). Ninety-one human, animal, and environmental S. brasiliensis isolates from different Brazilian geographic regions (South, Southeast, Midwest and Northeast) andtwo isolates from Paraguay were sequenced. The loci that presented the highest nucleotide diversity (π) were selected for the MLST scheme. Among the 16 studied genetic loci, four presented increased π value and were able to distinguish all S. brasiliensis isolates into seven distinct haplotypes. The PCR conditions were standardized for four loci. Some of the obtained haplotypes were associated with the geographic origin of the strains. This study presents an important advance in the understanding of this important agent of sporotrichosis in Brazil. It significantly increased the discriminatory power for genotyping of S. brasiliensis isolates, and enabled new contributions to the epidemiological studies of this human and animal pathogen in Brazil and in other countries. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade de Brasília. Faculdade de Medicina. Núcleo de Medicina Tropical. Brasília, DF, Brasil. | en_US |
Affilliation | Westerdijk Fungal Biodiversity Institute. Utrecht. Netherlands / Molecular Mycology Research Laboratory. Centre for Infectious Diseases and Microbiology. Westmead Clinical School. Sydney Medical School. Faculty of Medicine and Health. Sydney Infectious Diseases Institute. University of Sydney. Westmead Hospital. Research and Education Network. Westmead Institute for Medical Research. Sydney, Australia. | en_US |
Affilliation | Molecular Mycology Research Laboratory. Centre for Infectious Diseases and Microbiology. Westmead Clinical School. Sydney Medical School. Faculty of Medicine and Health. Sydney Infectious Diseases Institute. University of Sydney. Westmead Hospital. Research and Education Network. Westmead Institute for Medical Research. Sydney, Australia. | en_US |
Affilliation | Universidade Federal do Rio Grande. Faculdade de Medicina. Laboratório de Micologia e Programa de Pós-Graduação em Ciências da Saúde. Rio Grande, RS, Brasil. | en_US |
Affilliation | Universidade Federal do Rio Grande. Faculdade de Medicina. Laboratório de Micologia e Programa de Pós-Graduação em Ciências da Saúde. Rio Grande, RS, Brasil. | en_US |
Affilliation | Ministerio de Salud Pública y Bienestar Social. Centro de Especialidades Dermatologicas. Paraguay. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal da Paraíba. Departamento de Medicina Veterinária. João Pessoa, PB, Brasil. | en_US |
Affilliation | Universidade Federal da Paraíba. Departamento de Medicina Veterinária. João Pessoa, PB, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Sporotrichosis | en_US |
Subject | Epidemiology | en_US |
Subject | Genetic Variability | en_US |
Subject | Molecular Typing | en_US |
e-ISSN | 1096-0937 | |
Embargo date | 2030-12-31 | |