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https://www.arca.fiocruz.br/handle/icict/62800
METHOTREXATE AND NON-SURGICAL PERIODONTAL TREATMENT CHANGE THE ORAL–GUT MICROBIOTA IN RHEUMATOID ARTHRITIS: A PROSPECTIVE COHORT STUDY
Author
Rezende Oliveira, Sicília
Arruda, José Alcides Almeida de
Corrêa, Jôice Dias
Carvalho, Valessa Florindo
Medeiros, Julliane Dutra
Schneider, Ayda Henriques
Machado, Caio Cavalcante
Duffles, Letícia Fernanda
Fernandes, Gabriel da Rocha
Calderaro, Débora Cerqueira
Taba Júnior, Mario
Abreu, Lucas Guimarães
Fukada, Sandra Yasuyo
Oliveira, Renê Donizeti Ribeiro
Louzada-Júnior, Paulo
Cunha, Fernando Queiroz
Silva, Tarcília Aparecida
Arruda, José Alcides Almeida de
Corrêa, Jôice Dias
Carvalho, Valessa Florindo
Medeiros, Julliane Dutra
Schneider, Ayda Henriques
Machado, Caio Cavalcante
Duffles, Letícia Fernanda
Fernandes, Gabriel da Rocha
Calderaro, Débora Cerqueira
Taba Júnior, Mario
Abreu, Lucas Guimarães
Fukada, Sandra Yasuyo
Oliveira, Renê Donizeti Ribeiro
Louzada-Júnior, Paulo
Cunha, Fernando Queiroz
Silva, Tarcília Aparecida
Affilliation
Department of Oral Surgery, Pathology and Clinical Dentistry. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Oral Surgery, Pathology and Clinical Dentistry. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Dentistry. Pontifical Catholic University. Belo Horizonte, MG, Brazil.
Department of Oral and Maxillofacial Surgery and Periodontology. School of Dentistry of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Biology. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Pharmacology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Division of Clinical Immunology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of BioMolecular Sciences. School of Pharmaceutical Science, University of São Paulo. Ribeirão Preto, SP, Brazil.
René Rachou Institure. Oswaldo Cruz Fundation. Belo Horizonte, MG, Brazil.
Department of Locomotor Apparatus, Faculty of Medicine. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Oral and Maxillofacial Surgery and Periodontology. School of Dentistry of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Child and Adolescent Oral Health. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of BioMolecular Sciences. School of Pharmaceutical Science, University of São Paulo. Ribeirão Preto, SP, Brazil.
Division of Clinical Immunology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Division of Clinical Immunology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Pharmacology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Oral Surgery, Pathology and Clinical Dentistry. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Oral Surgery, Pathology and Clinical Dentistry. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Dentistry. Pontifical Catholic University. Belo Horizonte, MG, Brazil.
Department of Oral and Maxillofacial Surgery and Periodontology. School of Dentistry of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Biology. Federal University of Juiz de Fora. Juiz de Fora, MG, Brazil.
Department of Pharmacology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Division of Clinical Immunology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of BioMolecular Sciences. School of Pharmaceutical Science, University of São Paulo. Ribeirão Preto, SP, Brazil.
René Rachou Institure. Oswaldo Cruz Fundation. Belo Horizonte, MG, Brazil.
Department of Locomotor Apparatus, Faculty of Medicine. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Oral and Maxillofacial Surgery and Periodontology. School of Dentistry of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Child and Adolescent Oral Health. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of BioMolecular Sciences. School of Pharmaceutical Science, University of São Paulo. Ribeirão Preto, SP, Brazil.
Division of Clinical Immunology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Division of Clinical Immunology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Pharmacology. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Oral Surgery, Pathology and Clinical Dentistry. School of Dentistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Abstract
This study evaluated the changes in the composition of oral-gut microbiota in patients with rheumatoid arthritis (RA) caused by methotrexate (MTX) and non-surgical periodontal treatment (NSPT). Assessments were performed at baseline (T0), 6 months after MTX treatment (T1), and 45 days after NSPT (T2). The composition of the oral and gut microbiota was assessed by amplifying the V4 region of the 16S gene from subgingival plaques and stools. The results of the analysis of continuous variables were presented descriptively and non-parametric tests and Spearman's correlation were adopted. A total of 37 patients (27 with periodontitis) were evaluated at T0; 32 patients (24 with periodontitis) at T1; and 28 patients (17 with periodontitis) at T2. MTX tended to reduce the alpha diversity of the oral-gut microbiota, while NSPT appeared to increase the number of different species of oral microbiota. MTX and NSPT influenced beta diversity in the oral microbiota. The relative abundance of oral microbiota was directly influenced by periodontal status. MTX did not affect the periodontal condition but modified the correlations that varied from weak to moderate (p < 0.05) between clinical parameters and the microbiota. MTX and NSPT directly affected the composition and richness of the oral-gut microbiota. However, MTX did not influence periodontal parameters.
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