Author | Rocha, Leonardo de Oliveira Siquara da | |
Author | Souza, Bruno Solano de Freitas | |
Author | Coletta, Ricardo Della | |
Author | Lambert, Daniel W. | |
Author | Rocha, Clarissa A. Gurgel | |
Access date | 2024-02-21T14:27:15Z | |
Available date | 2024-02-21T14:27:15Z | |
Document date | 2023 | |
Citation | ROCHA, Leonardo de Oliveira Siquara da et al. Mapping cell-in-cell structures in oral squamous cell carcinoma. Cells, v. 12, n. 19, p. 1-15, 2023. | en_US |
ISSN | 1097-4172 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/62715 | |
Sponsorship | Academy of Medical Sciences/Newton Advanced Fellowship Grant.
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Program for Excellence in Research of the Oswaldo Cruz Foundation (PROEP-FIOCRUZ-BA). | en_US |
Language | eng | en_US |
Publisher | Elsevier | en_US |
Rights | open access | en_US |
Subject in Portuguese | Esferoides tumorais | en_US |
Subject in Portuguese | Carcinoma espinocelular oral | en_US |
Subject in Portuguese | Canibalismo celular | en_US |
Subject in Portuguese | Estruturas célula-em-célula | en_US |
Subject in Portuguese | Fibroblastos associados ao câncer | en_US |
Title | Mapping cell-in-cell structures in oral squamous cell carcinoma | en_US |
Type | Article | en_US |
DOI | 10.3390/cells12192418 | |
Abstract | Cell-in-cell (CIC) structures contribute to tumor aggressiveness and poor prognosis in oral squamous cell carcinoma (OSCC). In vitro 3D models may contribute to the understanding of the underlying molecular mechanisms of these events. We employed a spheroid model to study the CIC structures in OSCC. Spheroids were obtained from OSCC (HSC3) and cancer-associated fibroblast (CAF) lines using the Nanoshuttle-PLTM bioprinting system (Greiner Bio-One). Spheroid form, size, and reproducibility were evaluated over time (EvosTM XL; ImageJ version 1.8). Slides were assembled, stained (hematoxylin and eosin), and scanned (Axio Imager Z2/VSLIDE) using the OlyVIA System (Olympus Life Science) and ImageJ software (NIH) for cellular morphology and tumor zone formation (hypoxia and/or proliferative zones) analysis. CIC occurrence, complexity, and morphology were assessed considering the spheroid regions. Well-formed spheroids were observed within 6 h of incubation, showing the morphological aspects of the tumor microenvironment, such as hypoxic (core) and proliferative zone (periphery) formation. CIC structures were found in both homotypic and heterotypic groups, predominantly in the proliferative zone of the mixed HSC3/CAF spheroids. “Complex cannibalism” events were also noted. These results showcase the potential of this model in further studies on CIC morphology, formation, and relationship with tumor prognosis. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto D’Or de Pesquisa e Ensino (IDOR). Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Estadual de Campinas. Faculdade de Odontologia. Departamento de Diagnóstico Oral. Piracicaba, SP, Brasil / Universidade Estadual de Campinas. Faculdade de Odontologia. Programa de Pós-Graduação em Biologia Oral. Piracicaba, SP, Brasil. | en_US |
Affilliation | School of Clinical Dentistry. The University of Sheffield. Sheffield, UK. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Salvador, BA, Brasil / Instituto D’Or de Pesquisa e Ensino (IDOR). Salvador, BA, Brasil/ Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil. | en_US |
Subject | Tumor spheroids | en_US |
Subject | Oral squamous cell carcinoma | en_US |
Subject | Cell cannibalism | en_US |
Subject | Cell-in-cell structures | en_US |
Subject | Cancer-associated fibroblasts | en_US |
DeCS | Esteroides | en_US |
DeCS | Neoplasias | en_US |
DeCS | Carcinoma | en_US |
DeCS | Canibalismo | en_US |
DeCS | Formação de célula em célula | en_US |
DeCS | Fibroblastos | en_US |