Author | Campos, Gabriel Montenegro de | |
Author | La-Roque, Debora Glenda Lima de | |
Author | Lima, Alex Ranieri Jerônimo | |
Author | Zucherato, Victória Simionatto | |
Author | Carvalho, Eneas de | |
Author | Lima, Loyze Paola Oliveira de | |
Author | Cattony Neto, Pedro de Queiroz | |
Author | Santos, Murilo Marconi Dos | |
Author | Ciccozzi, Massimo | |
Author | Giovanetti, Marta | |
Author | Haddad, Rodrigo | |
Author | Alcantara, Luiz Carlos Júnior | |
Author | Elias, Maria Carolina | |
Author | Sampaio, Sandra Coccuzzo | |
Author | Covas, Dimas Tadeu | |
Author | Kashima, Simone | |
Author | Slavov, Svetoslav Nanev | |
Access date | 2024-01-17T19:32:19Z | |
Available date | 2024-01-17T19:32:19Z | |
Document date | 2023 | |
Citation | CAMPOS, Gabriel Montenegro de et al. Exploring Viral Metagenomics in Pediatric Patients with Acute Respiratory Infections: Unveiling Pathogens beyond SARS-CoV-2. Microorganisms, v. 11, n. 11, 2744, 2023. doi: 10.3390/microorganisms11112744. | en_US |
ISSN | 2076-2607 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/62268 | |
Language | eng | en_US |
Publisher | MDPI AG | en_US |
Rights | open access | en_US |
Title | Exploring Viral Metagenomics in Pediatric Patients with Acute Respiratory Infections: Unveiling Pathogens beyond SARS-CoV-2 | en_US |
Type | Article | en_US |
Abstract | The emergence of SARS-CoV-2 and the subsequent pandemic have prompted extensive diagnostic and clinical efforts to mitigate viral spread. However, these strategies have largely overlooked the presence of other respiratory viruses. Acute respiratory diseases in pediatric patients can be caused by a diverse range of viral agents, and metagenomics represents a powerful tool for their characterization. This study aimed to investigate the viral abundance in pediatric patients with acute respiratory symptoms who tested negative for SARS-CoV-2 during the Omicron pandemic wave. To achieve this, viral metagenomics and next-generation sequencing were employed on 96 nasopharyngeal swab samples, which were organized into 12 pools, with each pool consisting of eight individual samples. Metagenomic analysis revealed that the most prevalent viruses associated with acute disease in pediatric patients were respiratory syncytial virus (detected in all pools) and enteroviruses, which are known to cause significant morbidity and mortality in children. Additionally, clinically significant viruses such as mumps orthorubulavirus, human metapneumovirus, influenza A, and a wide array of human herpesviruses (1, 3-7) were identified. These findings highlight the extensive potential of viral metagenomics in identifying viruses other than SARS-CoV-2 that contribute to acute infections in children. Consequently, this methodology should garner clinical attention in terms of differential diagnosis and the development of public policies to address such conditions in the global pediatric population. | en_US |
Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Unit of Medical Statistics and Molecular Epidemiology. University Campus Bio-Medico of Rome. Rome, Italy. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil/Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy. | en_US |
Affilliation | University of Brasília. Brasilia, DF, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
Affilliation | Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil/Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil. | en_US |
Subject | acute infection | en_US |
Subject | enteroviruses | en_US |
Subject | next-generation sequencing | en_US |
Subject | respiratory syncytial virus | en_US |
Subject | respiratory viruses | en_US |
Subject | viral metagenomics | en_US |