Author | Araujo, Jéssica Mayra Mendes | |
Author | Monteiro, Joveliane Melo | |
Author | Silva, Douglas Henrique Dos Santos | |
Author | Veira, Amanda Karoline | |
Author | Silva, Maria Raimunda Chagas | |
Author | Ferraz, Fernanda Avelino | |
Author | Braga, Fábio H Ramos | |
Author | Siqueira Filho, Ezequias Pessoa de | |
Author | Monteiro, Andrea de Souza | |
Access date | 2024-01-16T19:35:40Z | |
Available date | 2024-01-16T19:35:40Z | |
Document date | 2023 | |
Citation | ARAUJO, Jéssica Mayra Mendes et al. Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity. Antibiotics, v. 12, n. 12, 1686, 2023. doi: 10.3390/antibiotics12121686. | en_US |
ISSN | 2079-6382 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/62209 | |
Language | eng | en_US |
Publisher | MDPI AG | en_US |
Rights | open access | en_US |
Title | Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity | en_US |
Type | Article | en_US |
Abstract | Surface-active compounds (SACs) of microbial origin are an active group of biomolecules with potential use in the formulation of emulsions. In this sense, the present study aimed to isolate and select yeasts from fruits that could produce SACs for essential oil emulsions. The Candida krusei M4CK was isolated from the Byrsonima crassifolia fruit to make SACs. This emulsification activity (E24) was equal to or greater 50% in all carbon sources, such as olive oil, sunflower oil, kerosene, hexane, and hexadecane. E24 followed exponential growth according to the growth phase. The stability of emulsions was maintained over a wide range of temperatures, pH, and salinity. The OMBE4CK (melaleuca essential oil emulsion) had better and more significant inhibitory potential for biofilm reduction formation. In addition, bioemulsifier BE4CK alone on Escherichia coli and Pseudomonas aeruginosa biofilm showed few effective results, while there was a significant eradication for Staphylococcus aureus biofilms. The biofilms formed by S. aureus were eradicated in all concentrations of OMBE4CK. At the same time, the preformed biofilm by E. coli and P. aeruginosa were removed entirely at concentrations of 25 mg/mL, 12.5 mg/mL, and 6.25 mg/mL. The results show that the bioemulsifier BE4CK may represent a new potential for antibiofilm application. | en_US |
Affilliation | BIONORTE. Rede de Biodiversidade e Biotecnologia da Amazônia Legal. São Luís, MA, Brazil/CEUMA. Laboratório de Microbiologia Aplicada. São Luís, MA, Brazil. | en_US |
Affilliation | BIONORTE. Rede de Biodiversidade e Biotecnologia da Amazônia Legal. São Luís, MA, Brazil/CEUMA. Laboratório de Microbiologia Aplicada. São Luís, MA, Brazil. | en_US |
Affilliation | Universidade CEUMA. Laboratório de Microbiologia Aplicada. São Luís, MA, Brazil. | en_US |
Affilliation | Universidade CEUMA. Laboratório de Microbiologia Aplicada. São Luís, MA, Brazil. | en_US |
Affilliation | Universidade CEUMA. Laboratório de Ciências do Ambiente. São Luís, MA, Brazil. | en_US |
Affilliation | Universidade CEUMA. Laboratório de Microbiologia Aplicada. São Luís, MA, Brazil. | en_US |
Affilliation | Universidade CEUMA. Laboratório de Ciências do Ambiente. São Luís, MA, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | BIONORTE. Rede de Biodiversidade e Biotecnologia da Amazônia Legal. São Luís, MA, Brazil/Universidade CEUMA. Laboratório de Microbiologia Aplicada. São Luís, MA, Brazil. | en_US |
Subject | Candida krusei | en_US |
Subject | antimicrobial activity | en_US |
Subject | bioemulsifier | en_US |
Subject | essential oil | en_US |