Author | Camandaroba, Edson Luiz Paes | |
Author | Reis, Eliana Almeida Gomes | |
Author | Reis, Mitermayer Galvão dos | |
Author | Andrade, Sonia Gumes | |
Access date | 2013-01-15T17:45:01Z | |
Available date | 2013-01-15T17:45:01Z | |
Document date | 2006 | |
Citation | CAMANDAROBA, E. L. P. et al. Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection. Memórias do Instituto Oswaldo Cruz, v. 101, n. 6, p. 613-615, sept. 2006. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/6197 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection | pt_BR |
Type | Article | pt_BR |
Abstract | Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Colombian strain clones | pt_BR |
Subject | Schizodeme | pt_BR |
DeCS | DNA de Cinetoplasto/genética | pt_BR |
DeCS | Trypanosoma cruzi/genética | pt_BR |
DeCS | Doença Aguda | pt_BR |
DeCS | Doença de Chagas/parasitologia | pt_BR |
DeCS | Células Clonais | pt_BR |
DeCS | Colômbia | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Polimorfismo de Fragmento de Restrição | pt_BR |
DeCS | Trypanosoma cruzi/enzimologia | pt_BR |
DeCS | Trypanosoma cruzi/patogenicidade | pt_BR |
DeCS | Virulência | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Camundongos | pt_BR |