Author | Assis, Gabriela Maíra Pereira de | |
Author | Alvarenga, Denise Anete Madureira de | |
Author | Souza, Luisa Braga e | |
Author | Sánchez-Arcila, Juan Camilo | |
Author | Silva, Eduardo Fernandes e | |
Author | Pina-Costa, Anielle de | |
Author | Gonçalves, Gustavo Henrique Pereira | |
Author | Junior Souza, Júlio César de | |
Author | Nunes, Ana Julia Dutra | |
Author | Pissinatti, Alcides | |
Author | Moreira, Silvia Bahadian | |
Author | Torres, Leticia de Menezes | |
Author | Costa, Helena Lott | |
Author | Tinoco, Herlandes da Penha | |
Author | Pereira, Valéria do Socorro | |
Author | Soares, Irene da Silva | |
Author | Sousa, Taís Nóbrega de | |
Author | Ntumngia, Francis Babila | |
Author | Adams, John H. | |
Author | Kano, Flora Satiko | |
Author | Hirano, Zelinda Maria Braga | |
Author | Pratt-Riccio, Lilian Rose | |
Author | Daniel-Ribeiro, Cláudio Tadeu | |
Author | Ferreira, Joseli Oliveira | |
Author | Carvalho, Luzia Helena | |
Author | Brito, Cristiana Ferreira Alves de | |
Access date | 2023-11-07T18:19:48Z | |
Available date | 2023-11-07T18:19:48Z | |
Document date | 2023 | |
Citation | ASSIS, Gabriela Maíra Pereira de et al. IgM antibody responses against Plasmodium antigens in neotropical primates in the Brazilian Atlantic Forest. Frontiers in Cellular and Infection Microbiology, v. 13, p. 1-16, 2023. | en_US |
ISSN | 2235-2988 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/61076 | |
Language | eng | en_US |
Publisher | Frontiers Media | en_US |
Rights | open access | en_US |
Title | IgM antibody responses against Plasmodium antigens in neotropical primates in the Brazilian Atlantic Forest | en_US |
Type | Article | en_US |
DOI | 10.3389/fcimb.2023.1169552 | |
Abstract | Introduction: Zoonotic transmission is a challenge for the control and elimination of malaria. It has been recorded in the Atlantic Forest, outside the Amazon which is the endemic region in Brazil. However, only very few studies have assessed the antibody response, especially of IgM antibodies, in Neotropical primates (NP). Therefore, in order to contribute to a better understanding of the immune response in different hosts and facilitate the identification of potential reservoirs, in this study, naturally acquired IgM antibody responses against Plasmodium antigens were evaluated, for the first time, in NP from the Atlantic Forest.
Methods: The study was carried out using 154 NP samples from three different areas of the Atlantic Forest. IgM antibodies against peptides of the circumsporozoite protein (CSP) from different Plasmodium species and different erythrocytic stage antigens were detected by ELISA.
Results: Fifty-nine percent of NP had IgM antibodies against at least one CSP peptide and 87% against at least one Plasmodium vivax erythrocytic stage antigen. Levels of antibodies against PvAMA-1 were the highest compared to the other antigens. All families of NP showed IgM antibodies against CSP peptides, and, most strikingly, against erythrocytic stage antigens. Generalized linear models demonstrated that IgM positivity against PvCSP and PvAMA-1 was associated with PCR-detectable blood-stage malaria infection and the host being free-living. Interestingly, animals with IgM against both PvCSP and PvAMA-1 were 4.7 times more likely to be PCR positive than animals that did not have IgM for these two antigens simultaneously.
Discussion: IgM antibodies against different Plasmodium spp. antigens are present in NP from the Atlantic Forest. High seroprevalence and antibody levels against blood-stage antigens were observed, which had a significant association with molecular evidence of infection. IgM antibodies against CSP and AMA-1 may be used as a potential marker for the identification of NP infected with Plasmodium, which are reservoirs of malaria in the Brazilian Atlantic Forest. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | School of Natural Sciences. Molecular and Cell Biology Department. University of California. Merced, Merced, CA, USA. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Vice-diretoria de Ensino. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brazil / Universidade Federal Fluminense. Escola de Enfermagem Aurora de Afonso Costa. Departamento de Doenças infecciosas e Parasitárias. Niterói, RJ, Brazil. | en_US |
Affilliation | Fundação Universidade Regional de Blumenau. Blumenau, SC, Brazil / Centro de Pesquisas Biológicas de Indaial. Indaial, SP, Brazil. | en_US |
Affilliation | Fundação Universidade Regional de Blumenau. Blumenau, SC, Brazil. | en_US |
Affilliation | Fundação Universidade Regional de Blumenau. Blumenau, SC, Brazil / Centro de Pesquisas Biológicas de Indaial. Indaial, SP, Brazil / Programa de conservação do Bugio Ruivo. Perini Business Park. Joinville, SC, Brazil. | en_US |
Affilliation | Instituto Estadual do Ambiente. Centro de Primatologia do Rio de Janeiro. Guapimirim, RJ, Brazil / Centro Universitário Serra dos Órgãos. Teresópolis, RJ, Brazil. | en_US |
Affilliation | Instituto Estadual do Ambiente. Centro de Primatologia do Rio de Janeiro. Guapimirim, RJ, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Prefeitura de Belo Horizonte. Fundação de Parques Municipais e Zoobotânica. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Prefeitura de Belo Horizonte. Fundação de Parques Municipais e Zoobotânica. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, FL, USA. | en_US |
Affilliation | Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, FL, USA. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Universidade Regional de Blumenau. Blumenau, SC, Brazil/Centro de Pesquisas Biológicas de Indaial. Indaial, SP, Brazil / Programa de conservação do Bugio Ruivo. Perini Business Park. Joinville, SC, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da malária. Belo Horizonte, MG, Brazil. | en_US |
Subject | Malaria | |
Subject | Plasmodium | |
Subject | IgM antibodies | |
Subject | Neotropical primates | |
Subject | Atlantic forest | |
Subject | Preerythrocytic stage antigen | |
Subject | Erythrocytic stage antigens | |